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Structural insights into the EthR-DNA interaction using native mass spectrometry.利用基质辅助激光解吸电离飞行时间质谱对EthR-DNA相互作用的结构洞察
Chem Commun (Camb). 2017 Mar 23;53(25):3527-3530. doi: 10.1039/c7cc00804j.
2
EthR, a repressor of the TetR/CamR family implicated in ethionamide resistance in mycobacteria, octamerizes cooperatively on its operator.EthR是TetR/CamR家族的一种阻遏蛋白，与分枝杆菌对乙硫异烟胺的抗性有关，它在其操纵基因上协同形成八聚体。
Mol Microbiol. 2004 Jan;51(1):175-88. doi: 10.1046/j.1365-2958.2003.03809.x.
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The Mycobacterium tuberculosis transcriptional repressor EthR is negatively regulated by Serine/Threonine phosphorylation.结核分枝杆菌转录抑制剂 EthR 受丝氨酸/苏氨酸磷酸化的负调控。
Biochem Biophys Res Commun. 2014 Apr 18;446(4):1132-8. doi: 10.1016/j.bbrc.2014.03.074. Epub 2014 Mar 22.
4
Fragment Screening against the EthR-DNA Interaction by Native Mass Spectrometry.采用天然质谱法筛选与 EthR-DNA 相互作用的片段。
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Crystal structure of the TetR/CamR family repressor Mycobacterium tuberculosis EthR implicated in ethionamide resistance.与乙硫异烟胺耐药性相关的结核分枝杆菌EthR（TetR/CamR家族阻遏蛋白）的晶体结构。
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Integrated analysis of ethionamide resistance loci in Mycobacterium tuberculosis clinical isolates.结核分枝杆菌临床分离株中乙硫异烟胺耐药位点的综合分析。
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Ethionamide boosters: synthesis, biological activity, and structure-activity relationships of a series of 1,2,4-oxadiazole EthR inhibitors.乙硫异烟胺增效剂：一系列 1,2,4-噁二唑 EthR 抑制剂的合成、生物活性和构效关系。
J Med Chem. 2011 Apr 28;54(8):2994-3010. doi: 10.1021/jm200076a. Epub 2011 Apr 1.
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A fragment merging approach towards the development of small molecule inhibitors of Mycobacterium tuberculosis EthR for use as ethionamide boosters.一种用于开发结核分枝杆菌EthR小分子抑制剂作为乙硫异烟胺增强剂的片段合并方法。
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New ethionamide boosters and EthR2: structural and energetic analysis.新型乙硫异烟胺增效剂和 EthR2：结构和能量分析。
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Cyclic di-GMP regulates Mycobacterium tuberculosis resistance to ethionamide.环二鸟苷酸调控结核分枝杆菌对乙硫异烟胺的耐药性。
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Charging of DNA Complexes in Positive-Mode Native Electrospray Ionization Mass Spectrometry.正模式原生电喷雾电离质谱法中DNA复合物的带电情况
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The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA-TetR regulator complex.合成 RNA 适体与转录阻遏物 TetR 形成的复合物是操纵子 DNA-TetR 调节复合物的结构和功能上的孪生体。
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本文引用的文献

1
Monitoring of the retinoic acid receptor-retinoid X receptor dimerization upon DNA binding by native mass spectrometry.通过原生质谱法监测视黄酸受体-视黄醇X受体在与DNA结合时的二聚化作用。
Biophys Chem. 2016 Mar;210:2-8. doi: 10.1016/j.bpc.2015.10.006. Epub 2015 Oct 30.
2
Crucial components of Mycobacterium type II fatty acid biosynthesis (Fas-II) and their inhibitors.分枝杆菌II型脂肪酸生物合成（Fas-II）的关键成分及其抑制剂。
FEMS Microbiol Lett. 2014 Nov;360(2):87-99. doi: 10.1111/1574-6968.12597. Epub 2014 Oct 21.
3
Type III restriction endonucleases are heterotrimeric: comprising one helicase-nuclease subunit and a dimeric methyltransferase that binds only one specific DNA.III 型限制内切酶是异源三聚体：由一个解旋酶-核酸酶亚基和一个二聚体甲基转移酶组成，后者仅与一种特定的 DNA 结合。
Nucleic Acids Res. 2014 Apr;42(8):5139-50. doi: 10.1093/nar/gku122. Epub 2014 Feb 7.
4
Enthalpic factors override the polyelectrolyte effect in the binding of EGR1 transcription factor to DNA.焓因子在 EGR1 转录因子与 DNA 的结合中超过聚电解质效应。
J Mol Recognit. 2014 Feb;27(2):82-91. doi: 10.1002/jmr.2336.
5
Integrative modelling coupled with ion mobility mass spectrometry reveals structural features of the clamp loader in complex with single-stranded DNA binding protein.整合建模与离子淌度质谱联用揭示了与单链 DNA 结合蛋白复合物中夹取器的结构特征。
J Mol Biol. 2013 Nov 29;425(23):4790-801. doi: 10.1016/j.jmb.2013.04.006. Epub 2013 Apr 11.
6
Two decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry.通过电喷雾电离质谱法研究非共价生物分子组装体二十年。
J R Soc Interface. 2012 May 7;9(70):801-16. doi: 10.1098/rsif.2011.0823. Epub 2012 Feb 7.
7
Synthetic EthR inhibitors boost antituberculous activity of ethionamide.合成的EthR抑制剂可增强乙硫异烟胺的抗结核活性。
Nat Med. 2009 May;15(5):537-44. doi: 10.1038/nm.1950. Epub 2009 May 3.
8
Determination of refractive index increment ratios for protein-nucleic acid complexes by surface plasmon resonance.通过表面等离子体共振测定蛋白质-核酸复合物的折射率增量比
Anal Biochem. 2007 Sep 15;368(2):148-55. doi: 10.1016/j.ab.2007.06.016. Epub 2007 Jun 15.
9
Determining the stoichiometry and interactions of macromolecular assemblies from mass spectrometry.通过质谱法确定大分子组装体的化学计量和相互作用。
Nat Protoc. 2007;2(3):715-26. doi: 10.1038/nprot.2007.73.
10
What drives proteins into the major or minor grooves of DNA?是什么促使蛋白质进入DNA的大沟或小沟？
J Mol Biol. 2007 Jan 5;365(1):1-9. doi: 10.1016/j.jmb.2006.09.059. Epub 2006 Sep 27.

利用基质辅助激光解吸电离飞行时间质谱对EthR-DNA相互作用的结构洞察

Structural insights into the EthR-DNA interaction using native mass spectrometry.

作者信息

Shiu-Hin Chan Daniel, Seetoh Wei-Guang, McConnell Brendan N, Matak-Vinković Dijana, Thomas Sherine E, Mendes Vitor, Blaszczyk Michal, Coyne Anthony G, Blundell Tom L, Abell Chris

机构信息

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK.

Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK.

出版信息

Chem Commun (Camb). 2017 Mar 23;53(25):3527-3530. doi: 10.1039/c7cc00804j.

DOI:10.1039/c7cc00804j
PMID:28287239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5868724/
Abstract

EthR is a transcriptional repressor that increases Mycobacterium tuberculosis resistance to ethionamide. In this study, the EthR-DNA interaction has been investigated by native electrospray-ionization mass spectrometry for the first time. The results show that up to six subunits of EthR are able to bind to its operator.

摘要

EthR是一种转录阻遏物，可增强结核分枝杆菌对乙硫异烟胺的耐药性。在本研究中，首次通过原生电喷雾电离质谱法研究了EthR与DNA的相互作用。结果表明，多达六个EthR亚基能够与其操纵基因结合。