利用基质辅助激光解吸电离飞行时间质谱对EthR-DNA相互作用的结构洞察
Structural insights into the EthR-DNA interaction using native mass spectrometry.
作者信息
Shiu-Hin Chan Daniel, Seetoh Wei-Guang, McConnell Brendan N, Matak-Vinković Dijana, Thomas Sherine E, Mendes Vitor, Blaszczyk Michal, Coyne Anthony G, Blundell Tom L, Abell Chris
机构信息
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB21EW, UK.
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK.
出版信息
Chem Commun (Camb). 2017 Mar 23;53(25):3527-3530. doi: 10.1039/c7cc00804j.
Abstract
EthR is a transcriptional repressor that increases Mycobacterium tuberculosis resistance to ethionamide. In this study, the EthR-DNA interaction has been investigated by native electrospray-ionization mass spectrometry for the first time. The results show that up to six subunits of EthR are able to bind to its operator.
摘要
EthR是一种转录阻遏物，可增强结核分枝杆菌对乙硫异烟胺的耐药性。在本研究中，首次通过原生电喷雾电离质谱法研究了EthR与DNA的相互作用。结果表明，多达六个EthR亚基能够与其操纵基因结合。
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