Liu Xue, German Guy K
Department of Biomedical Engineering, Binghamton University.
Department of Biomedical Engineering, Binghamton University;
J Vis Exp. 2017 Mar 1(121):55336. doi: 10.3791/55336.
Stratum corneum (SC) is the most superficial skin layer. Its contact with the external environment means that this tissue layer is subjected to both cleansing agents and daily variations in ambient moisture; both of which can alter the water content of the tissue. Reductions in water content from severe barrier dysfunction or low humidity environments can alter SC stiffness and cause a build-up of drying stresses. In extreme conditions, these factors can cause mechanical rupture of the tissue. We have established a high throughput method of quantifying dynamic changes in the mechanical properties of SC upon drying. This technique can be employed to quantify changes in the drying behavior and mechanical properties of SC with cosmetic cleanser and moisturizer treatments. This is achieved by measuring dynamic variations in spatially resolved in-plane drying displacements of circular tissue samples adhered to an elastomer substrate. In-plane radial displacements acquired during drying are azimuthally averaged and fitted with a profile based on a linear elastic contractility model. Dynamic changes in drying stress and SC elastic modulus can then be extracted from the fitted model profiles.
角质层（SC）是皮肤最表层。它与外部环境接触意味着该组织层会受到清洁剂和环境湿度每日变化的影响；这两者都会改变组织的含水量。严重屏障功能障碍或低湿度环境导致的含水量降低会改变角质层的硬度，并导致干燥应力的积累。在极端情况下，这些因素会导致组织的机械破裂。我们建立了一种高通量方法来量化干燥过程中角质层机械性能的动态变化。该技术可用于量化使用化妆品清洁剂和保湿剂处理后角质层干燥行为和机械性能的变化。这是通过测量附着在弹性体基质上的圆形组织样本在空间分辨的平面内干燥位移的动态变化来实现的。干燥过程中获得的平面内径向位移进行方位平均，并根据线性弹性收缩模型拟合曲线。然后可以从拟合的模型曲线中提取干燥应力和角质层弹性模量的动态变化。
