Hibicke Meghan, Graham Martha A, Hayslett Renée L
Department of Pharmaceutical Sciences.
Exp Clin Psychopharmacol. 2017 Apr;25(2):74-83. doi: 10.1037/pha0000119. Epub 2017 Mar 13.
Stress during times of rapid development is a risk factor for Major Depressive Disorder, a mood disorder that disproportionately affects women. We developed an adolescent chronic restraint stress (aCRS) protocol using female rats to address the impact of adolescent stress on female adult depressive-like behavior. Animals were divided into 4 treatment groups: not restrained:saline (NRSAL), not restrained:desipramine (NRDES), restrained:saline (RSAL), and restrained:desipramine (RDES). NRSAL and NRDES rats were housed in a separate colony room from RSAL and RDES rats. All animals were weighed and handled daily. Beginning postnatal day (PND) 34(±1), RSAL and RDES rats were restrained for 1 hour daily for 14 consecutive days. Beginning PND 55(±1), NRDES and RDES rats were given subcutaneous desipramine (5 mg/kg), which served as a positive control, daily for 14 consecutive days. During that same time period, NRSAL and RSAL rats were given subcutaneous saline daily. aCRS (RSAL and RDES) rats showed significantly attenuated weight gain compared with nonrestrained (NRSAL and NRDES) rats during the restraint period. Weight gain normalized after the final restraint session. Behavioral testing took place PND 68-69(±1), and included open field testing, the elevated plus maze, locomotor activity, and the forced swim test (FST). RSAL rats showed significantly more immobility in the FST versus all other groups, indicating depressive-like behavior. No differences between groups were observed in the other behavioral measures. These results indicate that aCRS elicits depressive-like behavioral characteristics in adult female rats without increasing anxiety-like behaviors. (PsycINFO Database Record
在快速发育时期的压力是重度抑郁症的一个风险因素，重度抑郁症是一种对女性影响尤为严重的情绪障碍。我们利用雌性大鼠开发了一种青少年慢性束缚应激（aCRS）方案，以研究青少年应激对成年雌性大鼠抑郁样行为的影响。将动物分为4个治疗组：未束缚：生理盐水（NRSAL）、未束缚：地昔帕明（NRDES）、束缚：生理盐水（RSAL）和束缚：地昔帕明（RDES）。NRSAL和NRDES组大鼠与RSAL和RDES组大鼠分置于不同的饲养室。所有动物每天称重并进行处理。从出生后第34天（±1天）开始，RSAL和RDES组大鼠连续14天每天被束缚1小时。从出生后第55天（±1天）开始，NRDES和RDES组大鼠每天皮下注射地昔帕明（5mg/kg）作为阳性对照，连续注射14天。在同一时期，NRSAL和RSAL组大鼠每天皮下注射生理盐水。在束缚期，aCRS组（RSAL和RDES）大鼠的体重增加明显低于未束缚组（NRSAL和NRDES）大鼠。在最后一次束缚实验后，体重增加恢复正常。行为测试在出生后第68 - 69天（±1天）进行，包括旷场试验、高架十字迷宫试验、运动活动测试和强迫游泳试验（FST）。与所有其他组相比，RSAL组大鼠在FST中表现出显著更多的不动时间，表明有抑郁样行为。在其他行为指标上未观察到组间差异。这些结果表明，aCRS可引发成年雌性大鼠的抑郁样行为特征，而不会增加焦虑样行为。（PsycINFO数据库记录）
