Kaliman A V, Krutilina A I, Kriukov V M
Mol Gen Mikrobiol Virusol. 1987 Oct(10):14-9.
The primary structures of two promoters (T5 P31A and T5 P31B) and transcription terminator from the region of bacteriophage T5 early genes D10-D15 were determined. Both promoters (62% of the phage T5 DNA physical map) are located as a tandem at 70 bp distance from each other and are typical for the promoter-consensus structure of DNA of bacteriophage T5 early promoters. The transcription terminator is located in the region of the BamHI site (67% of the phage T5 DNA physical map) and is arranged in good coincidence with the consensus structure of the already known rho-independent terminators of various organisms. The promotor T5 P31A and the terminator were tested for the level of galactokinase synthesis using galK gene-carrying plasmids and D-14C-galactose as a substrate for galactokinase.
测定了噬菌体T5早期基因D10 - D15区域的两个启动子（T5 P31A和T5 P31B）以及转录终止子的一级结构。两个启动子（占噬菌体T5 DNA物理图谱的62%）以串联形式位于彼此相距70 bp的位置，是噬菌体T5早期启动子DNA启动子共有结构的典型代表。转录终止子位于BamHI位点区域（占噬菌体T5 DNA物理图谱的67%），其排列与各种生物体中已知的不依赖ρ因子的终止子的共有结构高度吻合。使用携带galK基因的质粒和D - 14C - 半乳糖作为半乳糖激酶的底物，检测了启动子T5 P31A和终止子对半乳糖激酶合成水平的影响。
