Spotila L D, Rekosh D M, Boucher J M, LoVerde P T
Department of Microbiology, State University of New York, Buffalo 14214.
Mol Biochem Parasitol. 1987 Nov;26(1-2):17-20. doi: 10.1016/0166-6851(87)90125-3.
This report describes a method for the identification of the sex of Schistosoma mansoni cercariae using a cloned DNA probe which is female-specific. The probe is a 339 bp repeat; the sequence is presented. Cercariae from nine mono-miracidially infected snails were used in a double blind study. Mice were infected and the sex of adult worms observed. DNA was isolated from cercariae, digested with EcoRI, subjected to agarose gel electrophoresis, transferred to a matrix and hybridized with the cloned female-specific DNA probe. In all nine cases the sex of the cercariae as determined by DNA analysis agreed with the sex of the adult parasites.
本报告描述了一种使用克隆的雌性特异性DNA探针鉴定曼氏血吸虫尾蚴性别的方法。该探针是一个339bp的重复序列，已给出其序列。在一项双盲研究中使用了来自9只单尾蚴感染蜗牛的尾蚴。将小鼠感染并观察成虫的性别。从尾蚴中分离DNA，用EcoRI消化，进行琼脂糖凝胶电泳，转移到基质上并用克隆的雌性特异性DNA探针杂交。在所有9个案例中，通过DNA分析确定的尾蚴性别与成虫寄生虫的性别一致。
