单细胞绿藻中DUS16和DCL3对初级微小RNA的协同加工

Yamasaki Tomohito, Cerutti Heriberto

Division of Environmental Photobiology, National Institute for Basic Biology , Okazaki, Aichi, Japan.

School of Biological Science and Center for Plant Science Innovation, University of Nebraska-Lincoln , Lincoln, NE, USA.

Commun Integr Biol. 2017 Feb 6;10(1):e1280208. doi: 10.1080/19420889.2017.1280208. eCollection 2017.

We have previously reported that the RNA-binding protein Dull slicer 16 (DUS16) plays a key role in the processing of primary miRNAs (pri-miRNAs) in the unicellular green alga . In the present report, we elaborate on the interaction of DUS16 with Dicer-like 3 (DCL3) during pri-miRNA processing. Comprehensive analyses of small RNA libraries derived from mutant and wild-type algal strains allowed the de novo prediction of 35 pri-miRNA genes, including 9 previously unknown ones. The pri-miRNAs dependent on DUS16 for processing largely overlapped with those dependent on DCL3. Our findings suggest that DUS16 and DCL3 work cooperatively, presumably as components of a microprocessor complex, in the processing of the majority of pri-miRNAs in .

我们之前曾报道，RNA结合蛋白Dull slicer 16（DUS16）在单细胞绿藻初级miRNA（pri-miRNA）的加工过程中起关键作用。在本报告中，我们详细阐述了DUS16与Dicer样3（DCL3）在pri-miRNA加工过程中的相互作用。对来自突变型和野生型藻类菌株的小RNA文库进行的综合分析，使得能够从头预测35个pri-miRNA基因，其中包括9个先前未知的基因。依赖DUS16进行加工的pri-miRNA与依赖DCL3的pri-miRNA在很大程度上重叠。我们的研究结果表明，DUS16和DCL3在单细胞绿藻中大多数pri-miRNA的加工过程中协同工作，推测它们是微处理器复合体的组成部分。