Houghten R A, Pratt S M, Young E A, Brown H, Spann D R
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
NIDA Res Monogr. 1986;75:505-8.
A radioreceptor assay using [3H]2-beta-endorphin (B-EP) was used to examine binding in the brains of rats that had been exercised on a treadmill for 5 months. The binding of [3H]2-beta-EP to brain homogenates gave an average Kd of 0.830 +/- 0.055 nM for the controls and 0.826 + 0.022 nM for the runners, and respective Bmax's of 166 +/- 20 pM and 159 +/- 27 pM.
采用放射性受体分析法，使用[3H]2-β-内啡肽（B-EP）检测在跑步机上运动5个月的大鼠大脑中的结合情况。[3H]2-β-EP与脑匀浆的结合情况显示，对照组的平均解离常数（Kd）为0.830±0.055纳摩尔，跑步大鼠组为0.826±0.022纳摩尔，各自的最大结合容量（Bmax）分别为166±20皮摩尔和159±27皮摩尔。
