Raval Ishan H, Das Keshob C, Haldar Soumya
Academy of Scientific and Innovative Research, CSIR-CSMCRI, G. B. Marg, Bhavnagar, 364002, India.
CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar, Gujarat, 364002, India.
Environ Sci Pollut Res Int. 2017 Apr;24(11):10833-10845. doi: 10.1007/s11356-017-8555-y. Epub 2017 Mar 14.
The present study focuses on bacterial and selected heavy metal contaminations of economically important mullet fish (Mugil cephalus) collected from West coast of Gujarat, India. Molecular identification using 16S rRNA sequencing revealed the presence of Aeromonas veronii, A. mollusorum, A. cavae, A. bivalvum, and V.alginolyticus in the gill and the intestine along with some other non-pathogenic bacteria. Pathogenicity of different Aeromonas species was confirmed by hemolysin assay. Apart from pathogenicity, multidrug resistance pattern was also reported against some commonly used antibiotics. Heavy metal analysis of different parts such as ventral and dorsal muscles as well as gills of M. cephalus revealed maximum concentration of Pb (24.08 ± 4.40 mg/kg), Cd (8.25 ± 3.04 mg/kg), and Cu (33.67 ± 5.34 mg/kg), which were higher than the permissible limit. To the best of our knowledge, this is the first study analyzing different heavy metals and associated bacteria in M. cephalus fish in India. Further, the distribution of heavy metals in M. cephalus fish from other countries was also compared.
本研究聚焦于从印度古吉拉特邦西海岸采集的具有经济重要性的鲻鱼（ Mugil cephalus ）的细菌和特定重金属污染情况。使用 16S rRNA 测序进行分子鉴定发现，鳃和肠道中存在维氏气单胞菌、软体动物气单胞菌、卡氏气单胞菌、双壳类气单胞菌和溶藻弧菌，以及一些其他非致病细菌。通过溶血素试验证实了不同气单胞菌属物种的致病性。除了致病性外，还报告了对一些常用抗生素的多重耐药模式。对鲻鱼不同部位（如腹侧和背侧肌肉以及鳃）的重金属分析显示，铅（24.08 ± 4.40 毫克/千克）、镉（8.25 ± 3.04 毫克/千克）和铜（33.67 ± 5.34 毫克/千克）的浓度最高，均高于允许限值。据我们所知，这是印度首次对鲻鱼中的不同重金属和相关细菌进行分析的研究。此外，还比较了其他国家鲻鱼中重金属的分布情况。
