将¹²⁵I-人β-内啡肽与具有μ型或δ型阿片样物质结合位点的细胞系进行亲和交联。
Affinity crosslinking of 125I-human beta-endorphin to cell lines possessing either mu or delta type opioid binding sites.
作者信息
Keren O, Gioannini T L, Hiller J M, Simon E J
机构信息
Dept. of Psychiatry and Pharmacology, New York Univ. Med. Ctr, New York 10016.
出版信息
NIDA Res Monogr. 1986;75:89-92.
PMID:2829014
Abstract
Affinity crosslinking of human 125I-beta-Endorphin to cell lines possessing either mu or delta binding sites was carried out. Autoradiography of SDS-PAGE gels from these crosslinked cell lines revealed that these two sites contain major peptide subunits that differ in molecular size. This confirms our earlier finding in mammalian brain which demonstrated separate and distinct subunits for mu and delta opioid receptors.
摘要
进行了人125I-β-内啡肽与具有μ或δ结合位点的细胞系的亲和交联。对这些交联细胞系的SDS-PAGE凝胶进行放射自显影显示，这两个位点包含分子大小不同的主要肽亚基。这证实了我们早期在哺乳动物大脑中的发现，该发现证明了μ和δ阿片受体有各自独立且不同的亚基。
Zotero 插件