Yeung C W, Doyle S, Ellard S
Playfair Neuroscience Unit, Toronto Western Hospital, Ont., Canada.
Neurosci Lett. 1988 Jan 22;84(2):213-8. doi: 10.1016/0304-3940(88)90410-7.
125I-[D-Ala2,p-N3-Phe4-Met5]Enkephalin was used to investigate the opioid receptor from brain membranes of adult (6 months old) and fetal (16 days gestation) mice. The photolabeled membranes were analyzed by sodium dodecyl sulfate gel electrophoresis. A 46,000-Da protein was specifically photo-labeled in the adult mouse brain membranes. The photolabeling of this protein was inhibited in the presence of [D-Ala2,Met5]enkephalin and a peptide ligand specific for the mu-opioid receptor. No specific labeling of any protein was detected in the brain membranes of the fetal mice. This apparent lack of the opioid receptor in the fetal mouse brain suggests that the appearance of the opioid receptor in the adult mouse brain is subject to developmental regulation.
使用¹²⁵I-[D-丙氨酸²，对叠氮苯丙氨酸⁴，甲硫氨酸⁵]脑啡肽研究成年（6个月大）和胎儿（妊娠16天）小鼠脑膜中的阿片受体。通过十二烷基硫酸钠凝胶电泳分析光标记的膜。在成年小鼠脑膜中，一种46,000道尔顿的蛋白质被特异性光标记。在[D-丙氨酸²，甲硫氨酸⁵]脑啡肽和一种对μ-阿片受体特异的肽配体存在的情况下，该蛋白质的光标记受到抑制。在胎儿小鼠的脑膜中未检测到任何蛋白质的特异性标记。胎儿小鼠脑中阿片受体的这种明显缺乏表明成年小鼠脑中阿片受体的出现受到发育调控。
