脂质体布比卡因对关节软骨细胞的软骨毒性：初步研究结果。

Chondrotoxicity of Liposomal Bupivacaine in Articular Chondrocytes: Preliminary Findings.

作者信息

Shaw K Aaron, Johnson Peter C, Zumbrun Steve, Chuang Augustine H, Cameron Craig D

机构信息

Dwight D. Eisenhower Army Medical Center, Department of Orthopaedic Surgery, 300 E Hospital Road, Fort Gordon, GA 30905.

Dwight D. Eisenhower Army Medical Center, Department of Clinical Investigations, 300 E Hospital Road, Fort Gordon, GA 30905.

出版信息

Mil Med. 2017 Mar;182(S1):185-188. doi: 10.7205/MILMED-D-16-00079.

DOI:10.7205/MILMED-D-16-00079

PMID:28291471

Abstract

OBJECTIVE

The chondrotoxicity of local anesthetics has been previously recognized. Recent introduction of a liposomal formulation of bupivacaine has been found to significantly improve postoperative pain control but its effect on chondrocyte viability has yet to be investigated with this new formulation. We sought to assess the in vitro chondrotoxicity of liposomal bupivacaine.

METHODS

Chondrocytes were isolated from articular cartilage from fresh stifle joints and grown in culture medium. Cultured chondrocyte-derived cells (CDCs) were treated with 0.9% normal saline solution, 0.5%, 0.25%, and 0.13% bupivacaine and ropivacaine, 1.3% liposomal bupivacaine for 1 hour. Following treatment, cells were washed and incubated in media for 23 hours. The CDCs were then harvested and viability was assessed by flow cytometry using SYTOX green dead cell stain.

RESULTS

Treated CDCs demonstrated a dose-response effect for chondrocyte viability when treated with bupivacaine, ropivacaine, and liposomal bupivacaine. Liposomal bupivacaine demonstrated the highest chondrocyte viability following treatment. Ropivacaine demonstrated higher chondrocyte viability than bupivacaine.

CONCLUSION

Following 1 hour of treatment, liposomal bupivacaine demonstrated the highest chondrocyte viability. Chondrocyte viability was inversely proportional to anesthetic concentration.

摘要

目的

局部麻醉药的软骨毒性此前已得到确认。最近发现，布比卡因脂质体剂型的引入显著改善了术后疼痛控制，但其对软骨细胞活力的影响尚未在这种新剂型中进行研究。我们试图评估布比卡因脂质体的体外软骨毒性。

方法

从新鲜膝关节的关节软骨中分离软骨细胞，并在培养基中培养。将培养的软骨细胞衍生细胞（CDC）用0.9%生理盐水溶液、0.5%、0.25%和0.13%的布比卡因和罗哌卡因、1.3%的布比卡因脂质体处理1小时。处理后，洗涤细胞并在培养基中孵育23小时。然后收获CDC，并使用SYTOX绿色死细胞染色通过流式细胞术评估活力。