Lai Yen-Chun, Liang Chih-Ming, Hsu Shu-Chuan, Hsieh Ping-Heng, Hung Chun-Hsiung
Department of Environmental Engineering, National Chung Hsing University, 145 Xingda Rd., Taichung 40227, Taiwan.
Department of Environmental Engineering, National Chung Hsing University, 145 Xingda Rd., Taichung 40227, Taiwan.
J Biosci Bioeng. 2017 Jun;123(6):722-730. doi: 10.1016/j.jbiosc.2017.01.012. Epub 2017 Mar 11.
A purple non-sulfur bacterium, Rhodopseudomonas palustris G11, was isolated from an activated sludge plant that treats domestic wastewater. This isolation resulted in the effective accumulation of polyphosphate in cells upon reaching the stationary growth phase. However, when the carbon and/or energy source were/was removed, this bacterium released intracellular polyphosphate or poly-β-hydroxybutyrate to obtain energy to grow or maintain its growth. Furthermore, a novel photo-microbial fuel cell (PMFC) design was proposed. The unique capability of purple non-sulfur bacteria to capture light energy for polyphosphate accumulation was maximized. After R. palustris G11 accumulated considerable polyphosphate and was transferred to a fresh medium, the PMFC system exhibited a maximum voltage of approximately 0.03 V undt illumination. The chemical oxygen demand removal efficiency, Coulomb efficiency, and power density were 95.8%, 0.62%, and 0.15 mW/m, respectively. The test microorganisms converted most of the light energy in growth and caused the low power production. The microorganisms grew slowly and produced less power under dark conditions than under light illumination. However, these microorganisms used the previously stored polyphosphate or poly-β-hydroxybutyrate for electricity production when they were incubated in a growth-insufficient condition. This novel concept can be improved and optimized in the future for new PMFC applications, such as rechargeable cells, to treat wastewater and restore energy simultaneously.
从处理生活污水的活性污泥厂中分离出一株紫色非硫细菌——沼泽红假单胞菌G11。这种分离导致该细菌在进入稳定生长期时能在细胞内有效积累多聚磷酸盐。然而，当碳源和/或能源被去除时，这种细菌会释放细胞内的多聚磷酸盐或聚-β-羟基丁酸酯以获取能量来生长或维持其生长。此外，还提出了一种新型的光微生物燃料电池（PMFC）设计。紫色非硫细菌捕获光能用于多聚磷酸盐积累的独特能力得到了最大化发挥。在沼泽红假单胞菌G11积累了大量多聚磷酸盐并转移到新鲜培养基后，PMFC系统在光照下表现出约0.03 V的最大电压。化学需氧量去除效率、库仑效率和功率密度分别为95.8%、0.62%和0.15 mW/m。测试微生物在生长过程中转化了大部分光能，导致发电量较低。在黑暗条件下，微生物的生长速度比光照条件下慢且发电量更少。然而，当这些微生物在生长不足的条件下培养时，它们会利用先前储存的多聚磷酸盐或聚-β-羟基丁酸酯来发电。这种新概念在未来可针对新的PMFC应用进行改进和优化，如可充电电池，以同时处理废水和恢复能量。
