Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, China Agricultural University , Yuanmingyuan West Road 2, Beijing 100193, China.
J Agric Food Chem. 2017 Apr 19;65(15):3096-3102. doi: 10.1021/acs.jafc.7b00086. Epub 2017 Apr 7.
Research on the enantioselective behavior of chiral pesticides on amphibians has received growing attention, because amphibians are experiencing a population decline and amphibian metamorphosis shares many similarities with human fetal development. In this study, the enantioselective behavior of myclobutanil on Pelophylax nigromaculatus tadpole was studied. The antioxidant enzyme (SOD, GST) activities and malondialdehyde (MDA) content were investigated to assess the different toxic effects when tadpoles were exposed to myclobutanil enantiomers for 96 h. In the chronic exposure experiment, the bioaccumulation concentration of (-)-myclobutanil in tadpoles is significantly higher than that of (+)-myclobutanil, and the concentration of myclobutanil in tadpole intestine and liver was higher compared with other tissues. During the elimination experiment, about 95% of myclobutanil in tadpoles was eliminated within only 24 h. On the basis of these data, the enantiomeric differences should be taken into consideration in the risk assessment of myclobutanil.
手性农药在两栖动物上的对映体选择性行为研究受到越来越多的关注，因为两栖动物正面临着种群减少的问题，并且两栖动物的变态发育与人类胎儿的发育有许多相似之处。在这项研究中，研究了咪鲜胺对黑斑蛙蝌蚪的对映体选择性行为。通过检测抗氧化酶（SOD、GST）活性和丙二醛（MDA）含量，来评估当蝌蚪暴露于咪鲜胺对映体 96 h 时所产生的不同毒性作用。在慢性暴露实验中，（-）-咪鲜胺在蝌蚪体内的生物积累浓度明显高于（+）-咪鲜胺，且咪鲜胺在蝌蚪肠道和肝脏中的浓度高于其他组织。在消除实验中，蝌蚪体内的咪鲜胺在 24 h 内被消除了约 95%。基于这些数据，在咪鲜胺风险评估中应考虑对映体的差异。
