泡腾缓冲阿司匹林对人牙龈成纤维细胞前列腺素合成的影响。

Effect of effervescent buffered aspirin on prostaglandin synthesis by human gingival fibroblasts.

作者信息

ElAttar T M, Lin H S, Platt R D

机构信息

Laboratory of Hormone Research, University of Missouri School of Dentistry, Kansas City 64108.

出版信息

Prostaglandins Leukot Med. 1987 Oct;29(2-3):237-47. doi: 10.1016/0262-1746(87)90013-8.

DOI:10.1016/0262-1746(87)90013-8

PMID:2829238

Abstract

Cultured human gingival fibroblasts were incubated with 14C-arachidonic acid (AA) at 37 degrees C for 2 hours. The metabolites formed were extracted from the cell-free medium in methanol, separated and identified by thin layer chromatography, using two solvent systems that allowed resolution of cyclooxygenase and lipoxygenase products. The predominant cyclooxygenase products were PGE2 and 6-keto-PGF1 alpha. PGA2, PGF2 alpha, PGD2, 15-keto-PGE2, TXB2 were also detected in smaller amounts. No detectable radioactivity corresponding to lipoxygenase products 5-HETE, 12-HETE, and 15-HETE was found. Incubation of fibroblasts with effervescent buffered aspirin (EBA) (.02%, .04%, .06%), or sodium bicarbonate, citric acid and aspirin, individually, (in concentrations equivalent to those present in EBA) resulted in stimulation of synthesis of PGs except PGE2 which was inhibited by EBA and aspirin.

摘要

将培养的人牙龈成纤维细胞与14C - 花生四烯酸（AA）在37℃下孵育2小时。形成的代谢产物从无细胞培养基中用甲醇提取，通过薄层色谱法分离和鉴定，使用两种溶剂系统来分离环氧化酶和脂氧合酶产物。主要的环氧化酶产物是PGE2和6 - 酮 - PGF1α。还检测到少量的PGA2、PGF2α、PGD2、15 - 酮 - PGE2、TXB2。未发现与脂氧合酶产物5 - HETE、12 - HETE和15 - HETE相对应的可检测放射性。将成纤维细胞分别与泡腾缓冲阿司匹林（EBA）（0.02%、0.04%、0.06%）或碳酸氢钠、柠檬酸和阿司匹林（浓度与EBA中的浓度相当）孵育，结果除PGE2受到EBA和阿司匹林抑制外，其余前列腺素的合成均受到刺激。