Madison Joseph D, Berg Elizabeth A, Abarca Juan G, Whitfield Steven M, Gorbatenko Oxana, Pinto Adrian, Kerby Jacob L
Department of Biology, University of South Dakota Vermillion, SD, USA.
Centro de Investigación en Estructuras Microscópicas, Universidad de Costa Rica San Pedro de Montes de Oca, Costa Rica.
Front Microbiol. 2017 Feb 28;8:290. doi: 10.3389/fmicb.2017.00290. eCollection 2017.
Global amphibian declines and extinction events are occurring at an unprecedented rate. While several factors are responsible for declines and extinction, the fungal pathogen () has been cited as a major constituent in these events. While the effects of this chytrid fungus have been shown to cause broad scale population declines and extinctions, certain individuals and relict populations have shown resistance. This resistance has been attributed in part to the cutaneous bacterial microbiome. Here, we present the first study characterizing anti- bacterial isolates from amphibian populations in Costa Rica, including the characterization of two strains of presenting strong anti- activity. Transcriptome sequencing was utilized for delineation of shifts in gene expression of the two previously uncharacterized strains of grown in three different treatments comprising , heat-killed , and a no control. These results revealed up- and down-regulation of key genes associated with different metabolic and regulatory pathways. This information will be valuable in continued efforts to develop a bacterial-based approach for amphibian protection as well as providing direction for continued mechanistic inquiries of the bacterial anti- response.
全球两栖动物数量减少和灭绝事件正以前所未有的速度发生。虽然有几个因素导致了数量减少和灭绝，但真菌病原体（）被认为是这些事件的主要因素之一。虽然这种壶菌的影响已被证明会导致大规模的种群数量减少和灭绝，但某些个体和残余种群表现出了抗性。这种抗性部分归因于皮肤细菌微生物群。在此，我们展示了第一项对来自哥斯达黎加两栖动物种群的抗菌分离株进行表征的研究，包括对两株具有强抗菌活性的菌株的表征。转录组测序用于描绘在三种不同处理（包括活的、热灭活的和无处理对照）中生长的两株先前未表征的菌株的基因表达变化。这些结果揭示了与不同代谢和调节途径相关的关键基因的上调和下调。这些信息对于继续努力开发基于细菌的两栖动物保护方法以及为细菌抗菌反应的持续机制研究提供方向将是有价值的。
