Indrelid Stine, Kleiveland Charlotte, Holst René, Jacobsen Morten, Lea Tor
Research and Innovation, Østfold Hospital TrustKalnes, Norway; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life SciencesAas, Norway.
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences Aas, Norway.
Front Microbiol. 2017 Feb 28;8:320. doi: 10.3389/fmicb.2017.00320. eCollection 2017.
The prevalence of inflammatory bowel disease (IBD) has increased in Western countries during the course of the twentieth century, and is evolving to be a global disease. Recently we showed that a bacterial meal of a non-commensal, non-pathogenic methanotrophic soil bacterium, Bath prevents experimentally induced colitis in a murine model of IBD. The mechanism behind the effect has this far not been identified. Here, for the first time we show that , a soil bacterium adheres specifically to human dendritic cells, influencing DC maturation, cytokine production, and subsequent T cell activation, proliferation and differentiation. We characterize the immune modulatory properties of and compare its immunological properties to those of another Gram-negative gammaproteobacterium, the commensal K12, and the immune modulatory Gram-positive probiotic bacterium, GG . induces intermediate phenotypic and functional DC maturation. In a mixed lymphocyte reaction -primed monocyte-derived dendritic cells (MoDCs) enhance T cell expression of CD25, the γ-chain of the high affinity IL-2 receptor, supports cell proliferation, and induce a T cell cytokine profile different from both K12 and GG. Bath thus interacts specifically with MoDC, affecting MoDC maturation, cytokine profile, and subsequent MoDC directed T cell polarization.
在二十世纪，西方国家炎症性肠病（IBD）的患病率有所上升，并且正逐渐演变成一种全球性疾病。最近我们发现，一种非共生、非致病性的甲烷营养型土壤细菌——巴斯德杆菌的菌液，可预防IBD小鼠模型中实验性诱导的结肠炎。到目前为止，这种作用背后的机制尚未明确。在此，我们首次表明，这种土壤细菌能特异性地黏附于人类树突状细胞，影响树突状细胞的成熟、细胞因子产生以及随后的T细胞活化、增殖和分化。我们对该细菌的免疫调节特性进行了表征，并将其免疫特性与另一种革兰氏阴性γ-变形菌——共生菌K12以及具有免疫调节作用的革兰氏阳性益生菌——嗜热栖热放线菌GG进行了比较。该细菌可诱导树突状细胞出现中等程度的表型和功能成熟。在混合淋巴细胞反应中，经预处理的单核细胞衍生树突状细胞（MoDCs）可增强T细胞上CD25（高亲和力IL-2受体的γ链）的表达，支持细胞增殖，并诱导出与K12和GG均不同的T细胞细胞因子谱。因此，巴斯德杆菌与MoDC特异性相互作用，影响MoDC的成熟、细胞因子谱以及随后由MoDC介导的T细胞极化。
