Jichao Sun, Xinmin Han, Xianguo Ren, Dongqi Yin, Rongyi Zhou, Shuang Lei, Yue You, Yuchen Song, Jingnan Ying
The First Clinical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
Nanjing General Hospital of Nanjing Military Command, Nanjing, Jiangsu 210002, China.
Evid Based Complement Alternat Med. 2017;2017:2695903. doi: 10.1155/2017/2695903. Epub 2017 Feb 15.
The disturbed dopamine availability and brain-derived neurotrophic factor (BDNF) expression are due in part to be associated with attention deficit hyperactivity disorder (ADHD). In this study, we investigated the therapeutical effect of saikosaponin a (SSa) isolated from DC, against spontaneously hypertensive rat (SHR) model of ADHD. Methylphenidate and SSa were orally administered for 3 weeks. Activity was assessed by open-field test and Morris water maze test. Dopamine (DA) and BDNF were determined in specific brain regions. The mRNA or protein expression of tyrosine hydroxylase (TH), dopamine transporter (DAT), and vesicles monoamine transporter (VMAT) was also studied. Both MPH and SSa reduced hyperactivity and improved the spatial learning memory deficit in SHRs. An increased DA concentration in the prefrontal cortex (PFC) and striatum was also observed after treating with the SSa. The increased DA concentration may partially be attributed to the decreased mRNA and protein expression of DAT in PFC while SSa exhibited no significant effects on the mRNA expression of TH and VMAT in PFC of SHRs. In addition, BDNF expression in SHRs was also increased after treating with SSa or MPH. The obtained result suggested that SSa may be a potential drug for treating ADHD.
多巴胺可用性紊乱和脑源性神经营养因子（BDNF）表达部分归因于与注意力缺陷多动障碍（ADHD）相关。在本研究中，我们研究了从柴胡中分离出的柴胡皂苷a（SSa）对ADHD自发性高血压大鼠（SHR）模型的治疗效果。口服给予哌甲酯和SSa 3周。通过旷场试验和莫里斯水迷宫试验评估活动情况。在特定脑区测定多巴胺（DA）和BDNF。还研究了酪氨酸羟化酶（TH）、多巴胺转运体（DAT）和囊泡单胺转运体（VMAT）的mRNA或蛋白表达。MPH和SSa均降低了SHR的多动性并改善了空间学习记忆缺陷。用SSa治疗后，还观察到前额叶皮质（PFC）和纹状体中DA浓度增加。DA浓度增加可能部分归因于PFC中DAT的mRNA和蛋白表达降低，而SSa对SHR的PFC中TH和VMAT的mRNA表达无显著影响。此外，用SSa或MPH治疗后，SHR中的BDNF表达也增加。所得结果表明，SSa可能是治疗ADHD的潜在药物。
