Zhao Yanyan, Yu Feng, Liu Ruijuan, Dou Quanwen
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, the Chinese Academy of Sciences, Xining, 810008 China.
Mol Cytogenet. 2017 Mar 9;10:5. doi: 10.1186/s13039-017-0307-7. eCollection 2017.
L. is a turf grass and forage crop used worldwide. Being a facultative apomictic species, has a highly variable chromosome number. Chromosomal markers constitute a powerful tool for chromosome identification and for various aspects of genomic research. However, currently, no chromosomal markers are available for .
Four novel chromosome markers were isolated from a screen of Cot-1 DNA libraries, combined with fluorescence in situ hybridization (FISH) in . Three tandemly repetitive sequences (TR-1, TR-2, and TR-3) were characterized as subtelomeric. Monomers of 318 bp, 189 bp and 189 bp were identified in TR-1, TR-2, and TR-3, respectively. One tandemly repetitive sequence (CR-1) was shown to be centromeric or pericentromeric, and it had a monomer of 27 bp. The distribution patterns of TR-1, TR-2, and TR-3 were highly conserved across different cultivars and in the distantly related species, whereas CR-1 was conserved across different cultivars, but less conserved across species.
In this study, we report the identification and characterization of four novel chromosomal markers in . These chromosomal markers are powerful tools for accurate assessment of chromosome count, genomic and phylogenetic analyses, as well as studies of apomixis in
L. 是一种在全球范围内使用的草坪草和饲料作物。作为兼性无融合生殖物种，其染色体数目高度可变。染色体标记是染色体识别和基因组研究各个方面的有力工具。然而，目前尚无L. 的染色体标记。
从Cot-1 DNA文库筛选中分离出四个新型染色体标记，并结合荧光原位杂交（FISH）应用于L.。三个串联重复序列（TR-1、TR-2和TR-3）被鉴定为亚端粒序列。在TR-1、TR-2和TR-3中分别鉴定出318 bp、189 bp和189 bp的单体。一个串联重复序列（CR-1）被证明是着丝粒或着丝粒周围序列，其单体为27 bp。TR-1、TR-2和TR-3的分布模式在不同的L. 品种和远缘相关物种中高度保守，而CR-1在不同的L. 品种中保守，但在不同物种间保守性较低。
在本研究中，我们报告了在L. 中鉴定和表征的四个新型染色体标记。这些染色体标记是准确评估染色体数目、基因组和系统发育分析以及无融合生殖研究的有力工具。
