Edwards C K, Ghiasuddin S M, Schepper J M, Yunger L M, Kelley K W
Department of Animal Sciences, University of Illinois, Urbana 61801.
Science. 1988 Feb 12;239(4841 Pt 1):769-71. doi: 10.1126/science.2829357.
Macrophages can be activated to produce reactive oxygen intermediates, such as superoxide anion (O2-), which are responsible for intracellular killing of pathogenic microbes. Treatment with either native or recombinant somatotropin augmented the production of O2- by both peripheral blood-derived and alveolar macrophages stimulated with opsonized zymosan in vitro. This effect was abolished by prior treatment with an antibody specific for somatotropin. When either native or recombinant porcine somatotropin or native rat somatotropin was administered to hypophysectomized rats in vivo, activation of peritoneal macrophages, as measured by release of O2- in response to opsonized zymosan, was equivalent to that of macrophages from rats primed with the macrophage-activating factor interferon-gamma. Priming of macrophages in vivo was observed at physiologically relevant doses of somatotropin that caused a 10 to 40 percent increase in growth rate. Priming of mononuclear phagocytes for augmented production of reactive oxygen metabolites is a newly defined property of somatotropin.
巨噬细胞可被激活以产生活性氧中间体，如超氧阴离子（O2-），其负责在细胞内杀灭致病微生物。用天然或重组生长激素处理，均可增强体外经调理酵母聚糖刺激的外周血来源巨噬细胞和肺泡巨噬细胞产生O2-的能力。用生长激素特异性抗体预先处理可消除这种效应。当将天然或重组猪生长激素或天然大鼠生长激素给垂体切除的大鼠进行体内给药时，通过对经调理酵母聚糖刺激后O2-的释放来衡量，腹膜巨噬细胞的激活程度与用巨噬细胞激活因子γ干扰素致敏的大鼠的巨噬细胞相当。在生理相关剂量的生长激素下可观察到体内巨噬细胞的致敏，该剂量可使生长速率提高10%至40%。单核吞噬细胞产生活性氧代谢产物增强的致敏作用是生长激素新定义的特性。
