Song Won-Yong, Lee Sumin, Soh Moon-Soo
Department of Life Science, Pohang University of Science and Technology, Pohang, Republic of Korea.
Department of Integrative Bioscience and Biotechnology, College of Life Science, Sejong University, Seoul, Republic of Korea.
Methods Mol Biol. 2017;1573:41-46. doi: 10.1007/978-1-4939-6854-1_4.
1-Aminocyclopropane-1-carboxylic acid (ACC) is a biosynthetic precursor of ethylene. The movement of ACC across the plasma membrane (PM) has been implicated in various physiological contexts during environmental adaptation and differentiation in higher plants. A PM-localized transporter in Arabidopsis thaliana, LYSINE HISTIDINE TRANSPORTER1 (LHT1) participates in the uptake of ACC, implicating a class of amino-acid transporters in the transport of ACC. Here, we describe the method for assaying uptake of ACC into the plant cells using an Arabidopsis mesophyll protoplast system.
1-氨基环丙烷-1-羧酸（ACC）是乙烯的生物合成前体。在高等植物的环境适应和分化过程中，ACC跨质膜（PM）的转运参与了多种生理过程。拟南芥中一种定位于质膜的转运蛋白，赖氨酸组氨酸转运蛋白1（LHT1）参与了ACC的摄取，这表明一类氨基酸转运蛋白参与了ACC的转运。在这里，我们描述了一种使用拟南芥叶肉原生质体系统测定ACC进入植物细胞摄取量的方法。
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