筋骨草提取物的植物化学筛选、α-葡萄糖苷酶抑制、抗菌及抗氧化潜力

Phytochemical Screening, Alpha-Glucosidase Inhibition, Antibacterial and Antioxidant Potential of Ajuga bracteosa Extracts.

作者信息

Hafeez Kokab, Andleeb Saiqa, Ghousa Tahseen, Mustafa Rozina G, Naseer Anum, Shafique Irsa, Akhter Kalsoom

机构信息

Department of Chemistry, University of Azad Jammu and Kashmir, Muzaffarabad. Pakistan.

Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad, 13100. Pakistan.

出版信息

Curr Pharm Biotechnol. 2017;18(4):336-342. doi: 10.2174/1389201018666170313095033.

DOI:10.2174/1389201018666170313095033

PMID:28294059

Abstract

BACKGROUND

Ajuga bracteosa, a medicinal herb, is used by local community to cure a number of diseases such as inflammation, jaundice bronchial asthma, cancer and diabetes.

OBJECTIVES

The aim of present work was to evaluate the antioxidant potential, in vitro antidiabetic and antimicrobial effects of A. bracteosa.

METHODS

n-hexane, ethyl acetate, chloroform, acetone, methanol and aqueous extracts of Ajuga bracteosa roots, were prepared via maceration. Antibacterial activity was carried out by agar well diffusion method. Quantitative and qualitative phytochemical screening was done. The antioxidant activity was determined by iron (II) chelating activity, iron reducing power, DPPH, and ABTS free radical scavenging methods, Antidiabetic activity was evaluated through inhibition of α-glucosidase assay.

RESULTS

Phytochemical analysis showed the presence of phenols, flavonoids, tannins, saponins, quinines, terpenoids, xanthoproteins, glycosides, carbohydrates, steroids, phytosterols and amino acids. DPPH and ABTS potential values were recorded as 61.92% to 88.84% and 0.11% to 38.82%, respectively. Total phenolic and total flavonoid contents were expressed as gallic acid and rutin equivalents. Total iron content was expressed as FeSO4 equivalents. Chloroform and n-hexane extracts showed significant enzyme inhibition potential with IC50 values of 29.92 μg/ml and 131.7 μg/ml respectively. Aqueous extract showed maximum inhibition of E. coli, S. typhimurium, E. amnigenus, S. pyogenes, and S. aureus, (18.0±1.0 mm, 12.5±0.7 mm, 17.0±0.0 mm, 11.0±0.0 mm and 15.3±2.0 mm mm), respectively. Similarly, n-hexane extract showed maximum inhibition of E. coli, E. amnigenus, S. aureus (11.6±1.5 mm; 11.3±1.5 mm; 13.3±0.5 mm). This study also shows that n-hexane, chloroform, ethyl acetate and aqueous extracts of A. bracteosa root possess α-glucosidase inhibitory activities and therefore it may be used as hypoglycemic agents in the management of postprandial hyperglycemia.

CONCLUSION

Ajuga bracteosa root extracts may provide a basis for development of antioxidant, antimicrobial and antidiabetic drugs.

摘要

背景

药用植物苞叶筋骨草被当地社区用于治疗多种疾病，如炎症、黄疸、支气管哮喘、癌症和糖尿病。

目的

本研究旨在评估苞叶筋骨草的抗氧化潜力、体外抗糖尿病和抗菌作用。

方法

通过浸渍法制备苞叶筋骨草根的正己烷、乙酸乙酯、氯仿、丙酮、甲醇和水提取物。采用琼脂孔扩散法进行抗菌活性检测。进行了定量和定性的植物化学筛选。通过铁（II）螯合活性、铁还原能力、DPPH和ABTS自由基清除法测定抗氧化活性。通过抑制α-葡萄糖苷酶测定评估抗糖尿病活性。

结果

植物化学分析表明存在酚类、黄酮类、单宁、皂苷、奎宁、萜类、黄色蛋白、糖苷、碳水化合物、甾体、植物甾醇和氨基酸。DPPH和ABTS的潜在值分别记录为61.92%至88.84%和0.11%至38.82%。总酚和总黄酮含量分别以没食子酸和芦丁当量表示。总铁含量以硫酸亚铁当量表示。氯仿和正己烷提取物显示出显著的酶抑制潜力，IC50值分别为29.92μg/ml和131.7μg/ml。水提取物对大肠杆菌、鼠伤寒沙门氏菌、产氨短杆菌、化脓性链球菌和金黄色葡萄球菌的抑制作用最大，分别为（18.0±l.0mm、12.5±0.7mm、17.0±0.0mm、11.0±0.0mm和15.3±2.0mm）。同样，正己烷提取物对大肠杆菌、产氨短杆菌、金黄色葡萄球菌的抑制作用最大（11.6±1.5mm；11.3±1.5mm；13.3±0.5mm）。本研究还表明，苞叶筋骨草根的正己烷、氯仿、乙酸乙酯和水提取物具有α-葡萄糖苷酶抑制活性，因此可作为降血糖药物用于治疗餐后高血糖。