Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Phuthaditjhaba 9866, South Africa.
Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Phuthaditjhaba 9866, South Africa.
J Integr Med. 2018 Mar;16(2):132-140. doi: 10.1016/j.joim.2018.02.007. Epub 2018 Feb 12.
This study evaluates the phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity, and inhibitory activity against carbohydrate metabolism of extracts from Ocotea bullata stem bark.
Hexane, ethyl acetate, methanol and water were used to extract the air-dried sample. The phytochemical investigation and antioxidant assays were carried out on the extracts using standard procedures. The antidiabetic and anti-inflammatory potentials were evaluated using α-amylase, α-glucosidase and 5-lipoxygenase enzymes respectively. Vero cells were employed to determine the cytotoxicity of the extracts.
The ethyl acetate extract showed higher phenolic contents (8.97 mg/g gallic acid) while methanol displayed higher flavonoid (36.06 mg/g quercetin) and flavonol (153.44 mg/g rutin) contents than other extracts. Hexane extract had the greatest capacity to scavenge 1,1-diphenyl-2-picryl-hydrazyl (0.19 mg/mL), hydroxyl (25.77 mg/mL) and 2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (0.07 mg/mL) radicals, while ethyl acetate extract exhibited stronger inhibition (P < 0.05) against superoxide anion (0.41 mg/mL) and ferric ion-reducing power (2.36 mg/mL) compared to other extracts and standards. Aqueous extract (27.02 mg/mL) exhibited strong metal-chelating activity (P < 0.05) compared to other extracts and gallic acid. The aqueous extract demonstrated the greatest inhibition of α-glucosidase (1.45 mg/mL) and α-amylase (2.43 mg/mL) compared to other extracts and acarbose. There were no significant differences (P < 0.05) in half-maximum inhibitory concentration (IC) values of all tested extracts and indomethacin in the inhibition of 5-lipoxygenase activity. The aqueous extract was nontoxic to Vero cells with an IC value of 0.38 mg/mL.
O. bullata stem bark contains active phytochemicals with diverse pharmacological potentials that could be beneficial in managing diabetes and inflammation.
本研究评估了奥克蒂亚牛脂树皮提取物的植物化学成分、抗氧化和抗炎活性、细胞毒性以及对碳水化合物代谢的抑制活性。
使用正己烷、乙酸乙酯、甲醇和水从风干的样品中提取。使用标准程序对提取物进行植物化学研究和抗氧化测定。使用α-淀粉酶、α-葡萄糖苷酶和 5-脂氧合酶分别评估抗糖尿病和抗炎潜力。使用 Vero 细胞测定提取物的细胞毒性。
乙酸乙酯提取物表现出较高的酚含量（8.97mg/g 没食子酸），而甲醇提取物表现出较高的黄酮（36.06mg/g 槲皮素）和类黄酮醇（153.44mg/g 芦丁）含量。正己烷提取物对 1,1-二苯基-2-苦基肼（0.19mg/mL）、羟基（25.77mg/mL）和 2,2-氮杂双（3-乙基苯并噻唑啉-6-磺酸）（0.07mg/mL）自由基的清除能力最强，而乙酸乙酯提取物对超氧阴离子（0.41mg/mL）和铁离子还原能力（2.36mg/mL）的抑制作用（P<0.05）强于其他提取物和标准品。与其他提取物和没食子酸相比，水提取物（27.02mg/mL）具有较强的金属螯合活性。水提取物对α-葡萄糖苷酶（1.45mg/mL）和α-淀粉酶（2.43mg/mL）的抑制作用最强，与其他提取物和阿卡波糖相比。在抑制 5-脂氧合酶活性方面，所有测试提取物和吲哚美辛的半最大抑制浓度（IC）值均无显著差异（P<0.05）。水提取物对 Vero 细胞无毒，IC 值为 0.38mg/mL。
奥克蒂亚牛脂树皮含有具有多种药理潜力的活性植物化学成分，可能有益于糖尿病和炎症的治疗。
