Peluso R W, Moyer S A
Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Virology. 1988 Feb;162(2):369-76. doi: 10.1016/0042-6822(88)90477-1.
The viral proteins required for VSV RNA replication have been partially purified. With the use of monoclonal antibodies specific for the VSV N protein we have identified a putative N/NS complex present in the soluble protein fraction of infected cells. The complex is stable upon partial purification, contains the N and NS proteins in a 1:1 molar ratio, and has an elongated shape based on its hydrodynamic properties. Depletion of the N/NS complex from the infected cell soluble protein fraction results in the loss of the ability of this fraction to support RNA replication suggesting that the complex is required for this reaction. The ability to support viral genome RNA replication indeed cochromatographs with the N/NS protein complex through several steps of purification. Only the N protein of the N/NS complex appears to be bound to RNA during encapsidation with the release of NS protein.
水泡性口炎病毒（VSV）RNA复制所需的病毒蛋白已被部分纯化。利用针对VSV N蛋白的单克隆抗体，我们在受感染细胞的可溶性蛋白组分中鉴定出一种假定的N/NS复合物。该复合物在部分纯化后很稳定，以1:1的摩尔比包含N和NS蛋白，并且根据其流体动力学性质呈细长形状。从受感染细胞的可溶性蛋白组分中去除N/NS复合物会导致该组分支持RNA复制的能力丧失，这表明该复合物是此反应所必需的。支持病毒基因组RNA复制的能力确实在几个纯化步骤中与N/NS蛋白复合物共层析。在衣壳化过程中，随着NS蛋白的释放，似乎只有N/NS复合物中的N蛋白与RNA结合。
