Wei Rongfei, Guo Jing, Li Mengyuan, Yang Xingjiu, Zhu Ruimin, Huang Hao, Li Kejuan, Zhang Lingqiang, Gao Ran
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, Beijing, China.
Department of Inorganic Non-metallic Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, China.
FEBS Lett. 2017 Apr;591(8):1150-1158. doi: 10.1002/1873-3468.12624. Epub 2017 Apr 3.
Smad ubiquitination regulatory factor 1 (Smurf1) is a HECT-type E3 ubiquitin ligase that regulates several important signaling pathways, including the bone morphogenetic protein pathway and the transforming growth factor-beta (TGF-β) signaling pathway. However, the function of Smurf1 in cell cycle progression remains unclear. Here, we demonstrate that silencing of Smurf1 results in S phase arrest, confirming that Smurf1 is required for S phase progression. Furthermore, we demonstrate that Smurf1-mediated S phase progression is largely dependent on the ubiquitination-dependent degradation of Wee1. This study defines a novel role for Smurf1 in controlling S phase progression by promoting Wee1 degradation.
Smad泛素化调节因子1（Smurf1）是一种HECT型E3泛素连接酶，可调节多种重要信号通路，包括骨形态发生蛋白通路和转化生长因子-β（TGF-β）信号通路。然而，Smurf1在细胞周期进程中的功能仍不清楚。在此，我们证明Smurf1的沉默会导致S期阻滞，证实Smurf1是S期进程所必需的。此外，我们证明Smurf1介导的S期进程在很大程度上依赖于Wee1的泛素化依赖性降解。本研究确定了Smurf1在通过促进Wee1降解来控制S期进程中的新作用。
