Obom K M, Pogo B G
Center for Experimental Cell Biology, Mollie B. Roth Laboratory, Mount Sinai School of Medicine, City University of New York, NY 10029.
Virus Res. 1988 Jan;9(1):33-48. doi: 10.1016/0168-1702(88)90048-2.
To investigate if Shope fibroma virus (SFV), a leporipoxvirus that induces benign tumors in adult rabbits, can trigger the second step of carcinogenesis in vitro or malignant transformation, an already immortalized rabbit cell line (SIRC) was inoculated with ultraviolet-irradiated virus. The resulting cell transformants displayed the characteristic properties of the malignant phenotype: lack of infectious particles, low serum requirement, high efficiency of cloning, resistance to superinfection, presence of viral DNA sequences in the nucleus, expression of viral proteins and induction of tumors in rabbits. However, this transformation was not stable since in all cell lines studied, a loss of the malignant phenotype was recorded close to the 50th passage. To assess the oncogenic potential of SFV, NIH 3T3 cells were transfected with SFV DNA. The results of these experiments indicate that SFV DNA can induce the formation of foci in certain NIH 3T3 cell lines. Taken together these results support the notion that SFV can elicit the transformation of cells in vitro.
为了研究兔痘病毒（SFV），一种能在成年兔体内诱发良性肿瘤的兔痘病毒，是否能在体外引发致癌作用的第二步或恶性转化，已永生化的兔细胞系（SIRC）用紫外线照射过的病毒进行接种。产生的细胞转化体表现出恶性表型的特征特性：缺乏感染性颗粒、低血清需求、高效克隆、抗超感染、细胞核中存在病毒DNA序列、病毒蛋白表达以及在兔体内诱发肿瘤。然而，这种转化并不稳定，因为在所有研究的细胞系中，接近第50代时记录到恶性表型丧失。为了评估SFV的致癌潜力，用SFV DNA转染NIH 3T3细胞。这些实验结果表明，SFV DNA能在某些NIH 3T3细胞系中诱导灶形成。综合这些结果支持了SFV能在体外引发细胞转化的观点。
