两株肖普纤维瘤病毒的分子特征分析

Molecular characterization of two strains of Shope fibroma virus.

作者信息

Berkowitz E M, Pogo B G

出版信息

Virology. 1985 Apr 30;142(2):437-40. doi: 10.1016/0042-6822(85)90354-x.

Abstract

An isolate of Shope fibroma virus (SFV), designated Indiana (SFV-I), was previously described to be tumorigenic in vivo as SFV, cytocidal in vitro as the orthopoxviruses (vaccinia, rabbitpox, etc.), and to share antigenic determinants with SFV and vaccinia. The genetic relatedness of SFV-I to SFV and vaccinia was studied by means of Southern blotting and hybridization. The results indicated that SFV-I shares extensive DNA homology with vaccinia, but few common sequences with SFV. By contrast, SFV and vaccinia show no sequence homology. These findings suggest that SFV-I is an orthopoxvirus which carries some genetic information from the leporipoxviruses. Recombinants between two genera of poxviruses have not been reported before.

摘要

一种被命名为印第安纳（SFV-I）的肖普纤维瘤病毒（SFV）分离株，此前被描述为在体内具有致瘤性，如同SFV一样；在体外具有杀细胞性，如同正痘病毒（痘苗病毒、兔痘病毒等），并且与SFV和痘苗病毒共享抗原决定簇。通过Southern印迹法和杂交技术研究了SFV-I与SFV和痘苗病毒的遗传相关性。结果表明，SFV-I与痘苗病毒具有广泛的DNA同源性，但与SFV的共同序列很少。相比之下，SFV和痘苗病毒没有序列同源性。这些发现表明，SFV-I是一种正痘病毒，它携带了一些来自兔痘病毒属的遗传信息。痘病毒两个属之间的重组体此前尚未见报道。