Zeng Shuyun, Zhou Tao, Han Kai, Yang Yanci, Zhao Jianhua, Liu Zhan-Lin
Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Science, Northwest University, Xi'an, 710069, China.
Genes (Basel). 2017 Mar 15;8(3):103. doi: 10.3390/genes8030103.
is a non-parasitic genus in Orobanchaceae including six species mainly distributed in central and north China. Its phylogenetic position and infrageneric relationships remain uncertain due to potential hybridization and polyploidization. In this study, we sequenced and compared the complete chloroplast genomes of six species using Illumina sequencing technology to elucidate the interspecific variations. plastomes exhibited typical quadripartite and circular structures with good synteny of gene order. The complete genomes ranged from 153,622 bp to 154,055 bp in length, including 133 genes encoding 88 proteins, 37 tRNAs, and 8 rRNAs. Three genes (, , ) have potentially experienced positive selection. Plastome size variation of was mainly ascribed to the expansion and contraction of the border regions between the inverted repeat (IR) region and the single-copy (SC) regions. Despite of the conserved structure in plastomes, sequence variations provide useful phylogenetic information. Phylogenetic trees of 23 Lamiales species reconstructed with the complete plastomes suggested that was monophyletic and sister to the clade of and the parasitic taxa in Orobanchaceae. The interspecific relationships within were completely different with the previous studies. In future, population phylogenomic works based on plastomes are urgently needed to clarify the evolutionary history of .
是列当科中的一个非寄生属，包括6个物种，主要分布于中国中部和北部。由于可能存在杂交和多倍体化，其系统发育位置和属内关系仍不确定。在本研究中，我们使用Illumina测序技术对6个物种的完整叶绿体基因组进行了测序和比较，以阐明种间变异。叶绿体基因组呈现典型的四分体环状结构，基因顺序具有良好的共线性。完整基因组长度在153,622 bp至154,055 bp之间，包括133个基因，编码88种蛋白质、37个tRNA和8个rRNA。三个基因（，，）可能经历了正选择。的叶绿体基因组大小变异主要归因于反向重复（IR）区域和单拷贝（SC）区域之间边界区域的扩张和收缩。尽管叶绿体基因组结构保守，但序列变异提供了有用的系统发育信息。用完整叶绿体基因组重建的23个唇形目物种的系统发育树表明，是单系的，并且是和列当科寄生类群分支的姐妹群。内的种间关系与先前的研究完全不同。未来，迫切需要基于叶绿体基因组的群体系统发育基因组学工作来阐明的进化历史。
