Kuwahara K, Yoshimura Y, Isobe N
Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan.
Reprod Domest Anim. 2017 Aug;52(4):579-584. doi: 10.1111/rda.12948. Epub 2017 Mar 12.
The objective of this study was to compare the dynamics of innate immune components after intramammary infusion of Staphylococcus aureus (SA) under conditions of high oestrogen and high progesterone in goats. In one group ("E-group"), controlled internal drug release (CIDR) devices were inserted intravaginally from days -11 to -4. Prostaglandin F2α was administered immediately after removal of the CIDR device at day -3, and then oestradiol benzoate (E) was injected intramuscularly once a day from days -2 to 3. Heat-inactivated SA was then administered via intramammary infusion to the left udder at day 0, whilst only saline was infused to the right udder as a control. In a second group ("P-group"), CIDR devices were inserted intravaginally from days -3 to 7 and SA was infused at day 0 in the same way as in the E-group. The milk yield and the concentration of innate immune components (somatic cell count (SCC), lactoferrin (LF), S100A7 and goat ß-defensin 1 (GBD-1)) in the milk were measured. Milk yield decreased drastically in both SA and control udders in the E-group, whereas the P-group exhibited increased milk yield in both SA and control udders. SCC increased after SA infusion in both E- and P-groups, although it was higher in the E-group than in the P-group. There was no significant change in LF concentration in the E-group, but a decrease was observed in the P-group. Concentrations of S100A and GBD-1 were significantly increased after SA infusion in the E-group but not in the P-group. These results suggest that E enhances the innate immune response induced by SA in the goat mammary gland. This effect may be due to the reduction in milk yield and upregulation of innate immune components.
本研究的目的是比较在高雌激素和高孕酮条件下，山羊乳腺内注入金黄色葡萄球菌（SA）后先天免疫成分的动态变化。在一组（“E组”）中，从第-11天至-4天经阴道插入可控性内部药物释放（CIDR）装置。在第-3天取出CIDR装置后立即给予前列腺素F2α，然后从第-2天至第3天每天肌肉注射一次苯甲酸雌二醇（E）。然后在第0天通过乳腺内注入热灭活的SA至左侧乳房，而右侧乳房仅注入生理盐水作为对照。在第二组（“P组”）中，从第-3天至第7天经阴道插入CIDR装置，并在第0天以与E组相同的方式注入SA。测量了产奶量以及乳汁中先天免疫成分（体细胞计数（SCC）、乳铁蛋白（LF）、S100A7和山羊β-防御素1（GBD-1））的浓度。E组中SA和对照乳房的产奶量均大幅下降，而P组中SA和对照乳房的产奶量均增加。E组和P组在注入SA后SCC均增加，尽管E组的SCC高于P组。E组中LF浓度无显著变化，但P组中观察到LF浓度下降。E组在注入SA后S100A和GBD-1的浓度显著增加，而P组未增加。这些结果表明，E增强了山羊乳腺中SA诱导的先天免疫反应。这种作用可能归因于产奶量的减少和先天免疫成分的上调。
