巨型单层囊泡中金丝桃素大聚集体的形成——实验与建模
Formation of Large Hypericin Aggregates in Giant Unilamellar Vesicles-Experiments and Modeling.
作者信息
Joniova Jaroslava, Rebič Matúš, Strejčková Alena, Huntosova Veronika, Staničová Jana, Jancura Daniel, Miskovsky Pavol, Bánó Gregor
机构信息
Department of Biophysics, Institute of Physics, Faculty of Science, P.J. Šafárik University, Košice, Slovakia; Laboratory of Organometallic and Medicinal Chemistry, ISIC, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
Department of Biophysics, Institute of Physics, Faculty of Science, P.J. Šafárik University, Košice, Slovakia.
出版信息
Biophys J. 2017 Mar 14;112(5):966-975. doi: 10.1016/j.bpj.2017.01.019.
The incorporation of hypericin (Hyp) from aqueous solutions into giant unilamellar vesicle (GUV) membranes has been studied experimentally and by means of kinetic Monte Carlo modeling. The time evolution of Hyp fluorescence originating from Hyp monomers dissolved in the GUV membrane has been recorded by confocal microscopy and while trapping individual GUVs in optical tweezers. It was shown that after reaching a maximum, the fluorescence intensity gradually decreased toward longer times. Formation of oversized Hyp clusters has been observed on the GUV surface at prolonged time. A simplified kinetic Monte Carlo model is presented to follow the aggregation/dissociation processes of Hyp molecules in the membrane. The simulation results reproduced the basic experimental observations: the scaling of the characteristic fluorescence decay time with the vesicle diameter and the buildup of large Hyp clusters in the GUV membrane.
已通过实验和动力学蒙特卡罗建模研究了金丝桃素（Hyp）从水溶液掺入巨型单层囊泡（GUV）膜的过程。通过共聚焦显微镜并在光镊中捕获单个GUV，记录了源自溶解在GUV膜中的Hyp单体的Hyp荧光随时间的变化。结果表明，在达到最大值后，荧光强度随时间逐渐降低。长时间后，在GUV表面观察到形成了超大的Hyp簇。提出了一个简化的动力学蒙特卡罗模型来跟踪膜中Hyp分子的聚集/解离过程。模拟结果重现了基本的实验观察结果：特征荧光衰减时间与囊泡直径的比例关系以及GUV膜中大型Hyp簇的形成。
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