Department of Biomedical Engineering, Faculty of Fundamental Technical Problems, Wrocław University of Science and Technology, 50-377 Wrocław, Pl. Grunwaldzki 13, Poland.
Department of Biomedical Engineering, Faculty of Fundamental Technical Problems, Wrocław University of Science and Technology, 50-377 Wrocław, Pl. Grunwaldzki 13, Poland.
Chem Phys Lipids. 2018 May;212:88-95. doi: 10.1016/j.chemphyslip.2018.01.001. Epub 2018 Jan 31.
Giant unilamellar vesicles (GUVs) have become one of extensively studied biological bilayer models especially when investigating topological and mechanical properties of cell membranes. They are also used to visualize membrane-related phenomena. However, the method of preparation and the effects of parameters of preparation on the vesicular structure are extensively varied. Therefore, it is important to understand how the process of formation of GUVs influences the outcome population, as it can influence the outcome of the experiment that is planned. Therefore, in this study, we investigated the effects of protocol parameters of electroformation on properties of homogeneous population of POPC GUVs. The parameters investigated in this study are duration of electroformation, usage of electrodes and frequency of applied AC field and its voltage. The properties investigated, which can be used to describe GUV populations are average diameter of vesicle, the amount of lipid molecules in population, and structure of vesicles. According to our results, prolonged time (greater than 4 h) does not influence outcome; however, parameters of applied electrical field (voltage and frequency) did significantly influence the properties of obtained POPC GUV populations.
巨大单层囊泡（GUVs）已成为广泛研究的生物双层模型之一，特别是在研究细胞膜的拓扑和力学性质时。它们也用于可视化与膜相关的现象。然而，制备方法以及制备参数对囊泡结构的影响差异很大。因此，了解 GUV 形成过程如何影响群体结果非常重要，因为它可能会影响计划的实验结果。因此，在这项研究中，我们研究了电成形协议参数对均匀 POPC GUV 群体性质的影响。本研究中研究的参数是电成形的持续时间、电极的使用以及施加的交流电场的频率及其电压。可以用来描述 GUV 群体的研究性质包括囊泡的平均直径、群体中脂质分子的数量以及囊泡的结构。根据我们的结果，延长时间（大于 4 小时）不会影响结果；然而，施加的电场参数（电压和频率）确实显著影响了所获得的 POPC GUV 群体的性质。
