白细胞介素-28B rs12979860 C/T多态性与肝移植后的急性细胞排斥反应
Interleukin-28B rs12979860 C/T Polymorphism and Acute Cellular Rejection after Liver Transplantation.
作者信息
Fereidooni H, Azarpira N, Yaghobi R, Vahdati A, Malek-Hoseini S A
机构信息
Department of Physiology, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran.
Transplant Research Center, Stem Cell Research Institute, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Int J Organ Transplant Med. 2017;8(1):28-33. Epub 2017 Feb 1.
BACKGROUND
Interleukin-28 (IL-28B) rs12979860 C/T polymorphism is a known predictor of sustained virological response after antiviral treatment in hepatitis C. IL-28B affects the innate immune system as well as intrahepatic expression level of interferon-stimulated genes.
OBJECTIVE
To investigate the effect of recipient IL-28B polymorphism on occurrence of acute rejection after liver transplantation.
METHODS
140 liver allograft recipients were selected. Acute rejection episodes were recorded in 39 patients (AR group); the remaining had normal graft function (non-AR group). 70 normal subjects were also studied as the control group. The IL-28B rs12979860 was genotyped through PCR-RFLP method.
RESULTS
No significant difference was found between AR and non-AR groups in terms of genotype and allele frequency. However, the CC genotype was significantly (p<0.001) more frequent in patients than in the control group; the allele variants increased the risk of end-stage liver disease (OR: 2.60).
CONCLUSION
Liver damage in association with the carriage of IL-28B C allele is associated with a higher likelihood of developing cirrhosis.
背景
白细胞介素-28（IL-28B）rs12979860 C/T基因多态性是丙型肝炎抗病毒治疗后持续病毒学应答的已知预测指标。IL-28B影响先天性免疫系统以及干扰素刺激基因的肝内表达水平。
目的
探讨受体IL-28B基因多态性对肝移植后急性排斥反应发生的影响。
方法
选取140例肝移植受者。记录39例发生急性排斥反应的患者（AR组）；其余患者移植肝功能正常（非AR组）。另选取70例正常受试者作为对照组。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对IL-28B rs12979860进行基因分型。
结果
AR组与非AR组在基因型和等位基因频率方面无显著差异。然而，患者中CC基因型的频率显著高于对照组（p<0.001）；等位基因变异增加了终末期肝病的风险（比值比：2.60）。
结论
与携带IL-28B C等位基因相关的肝损伤与发生肝硬化的可能性较高有关。
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