Cai Hui, Yu Yongxin, Jin Miao, Pan Yingjie, Yan Shuling, Wang Yongjie
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai, China.
Arch Virol. 2017 Jul;162(7):2053-2059. doi: 10.1007/s00705-017-3325-1. Epub 2017 Mar 15.
The genome sequence of a rare recombinant norovirus (NoV) genotype obtained from clinical samples in China was determined using one-step reverse transcription PCR. It was identified as the GII.P7/GII.6 genotype using both phylogenetic and SimPlot analyses. A high degree of variability was observed in the P2 subdomain, especially in the B-loop structure. The recombination breakpoints of all available GII.P7/GII.6 strains were mapped to two different positions within the RdRp region, both of which were at least 40 nt upstream of the overlap of ORF1 and 2. The GII.P7/GII.6 genotype appears to have been circulating in Asia for at least 10 years.
利用一步法逆转录聚合酶链反应（PCR）测定了从中国临床样本中获得的一种罕见重组诺如病毒（NoV）基因型的基因组序列。通过系统发育分析和SimPlot分析，该病毒被鉴定为GII.P7/GII.6基因型。在P2亚结构域中观察到高度变异性，尤其是在B环结构中。所有可用的GII.P7/GII.6毒株的重组断点被定位到RdRp区域内的两个不同位置，这两个位置均位于开放阅读框1和2重叠区上游至少40个核苷酸处。GII.P7/GII.6基因型似乎已在亚洲传播至少10年。
