Izumi S, Sakurai H, Fujii T, Ikeda W, Tomino S
Department of Biology, Tokyo Metropolitan University, Japan.
Biochim Biophys Acta. 1988 Feb 28;949(2):181-8. doi: 10.1016/0167-4781(88)90081-4.
A major plasma protein, referred to as SP 1, exhibits sex- and stage-specific expression during the larval development of the silkworm, Bombyx mori. We have isolated a plasmid clone bearing a part of mRNA sequence coding for SP 1 and quantitated the amount of SP 1 mRNA by means of RNA blot hybridization using the cDNA probe. The developmental change in the amount of SP 1 mRNA in the fat body closely reflected that of the hemolymph concentration of SP 1, indicating that the biosynthesis of SP 1 is regulated in a sex- and developmental stage-specific fashion at the level of mRNA.
