Kirkegaard Julie, Rozlosnik Noemi
DTU Nanotech, Institut for Mikro- og Nanoteknologi, Ørsteds Plads, bygning 345Ø, DK-2800, Kgs. Lyngby, Denmark.
Methods Mol Biol. 2017;1572:55-70. doi: 10.1007/978-1-4939-6911-1_5.
In this chapter a detailed description of the fabrication and testing of an aptasensor for influenza A virus detection is given. The sensor chip is an all-polymer chip fabricated with screen-printed poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) electrodes. Chip substrates are made by CO laser cutting of Poly(methyl methacrylate) (PMMA) sheets. Influenza A virus specific aptamers are immobilized onto the electrodes by UV cross-linking. Impedance based measurements at a single frequency, measured over time, are used to detect the virus in a buffer solution.
本章详细描述了用于甲型流感病毒检测的适配体传感器的制备和测试。该传感器芯片是一种全聚合物芯片，由丝网印刷的聚（3,4-乙撑二氧噻吩）聚苯乙烯磺酸盐（PEDOT:PSS）电极制成。芯片基板通过CO激光切割聚甲基丙烯酸甲酯（PMMA）片材制成。甲型流感病毒特异性适配体通过紫外线交联固定在电极上。通过在单个频率下随时间进行的基于阻抗的测量来检测缓冲溶液中的病毒。
