用于DNA杂交事件电化学电容检测的微流控阵列芯片实验室

Microfluidic Arrayed Lab-On-A-Chip for Electrochemical Capacitive Detection of DNA Hybridization Events.

作者信息

Ben-Yoav Hadar, Dykstra Peter H, Bentley William E, Ghodssi Reza

机构信息

Department of Biomedical Engineering, Ben-Gurion University of the Negev, Beer Sheva, 8410501, Israel.

MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research, University of Maryland, College Park, MD, 20742, USA.

出版信息

Methods Mol Biol. 2017;1572:71-88. doi: 10.1007/978-1-4939-6911-1_6.

DOI:10.1007/978-1-4939-6911-1_6

PMID:28299682

Abstract

A microfluidic electrochemical lab-on-a-chip (LOC) device for DNA hybridization detection has been developed. The device comprises a 3 × 3 array of microelectrodes integrated with a dual layer microfluidic valved manipulation system that provides controlled and automated capabilities for high throughput analysis of microliter volume samples. The surface of the microelectrodes is functionalized with single-stranded DNA (ssDNA) probes which enable specific detection of complementary ssDNA targets. These targets are detected by a capacitive technique which measures dielectric variation at the microelectrode-electrolyte interface due to DNA hybridization events. A quantitative analysis of the hybridization events is carried out based on a sensing modeling that includes detailed analysis of energy storage and dissipation components. By calculating these components during hybridization events the device is able to demonstrate specific and dose response sensing characteristics. The developed microfluidic LOC for DNA hybridization detection offers a technology for real-time and label-free assessment of genetic markers outside of laboratory settings, such as at the point-of-care or in-field environmental monitoring.

摘要

一种用于DNA杂交检测的微流控电化学芯片实验室（LOC）装置已被开发出来。该装置包括一个3×3的微电极阵列，与双层微流控阀控操作系统集成在一起，该系统为微升体积样品的高通量分析提供了可控和自动化的能力。微电极表面用单链DNA（ssDNA）探针进行功能化，能够特异性检测互补的ssDNA靶标。这些靶标通过电容技术进行检测，该技术测量由于DNA杂交事件在微电极-电解质界面处的介电变化。基于传感模型对杂交事件进行定量分析，该模型包括对能量存储和耗散组件的详细分析。通过在杂交事件期间计算这些组件，该装置能够展示出特异性和剂量响应传感特性。所开发的用于DNA杂交检测的微流控LOC提供了一种技术，可在实验室环境之外，如在护理点或现场环境监测中，对遗传标记进行实时、无标记评估。