Huck Claudia, Barud Hernane da Silva, Basso Fernanda Gonçalves, Costa Carlos Alberto de Souza, Hebling Josimeri, Garcia Lucas da Fonseca Roberti
Department of Restorative Dentistry, Araraquara Dental School, UNESP - Universidade Estadual Paulista, Araraquara, SP, Brazil.
Medical-Chemistry Laboratory and Regenerative Medicine (QUIMMERA), Centro Universitário de Araraquara, Araraquara, SP, Brazil.
Braz Dent J. 2017 Jan-Feb;28(1):57-64. doi: 10.1590/0103-6440201701023.
This study aimed to evaluate the cytotoxicity of a calcium aluminate cement (EndoBinder) containing different radiopacifiers, Bi2O3, ZnO or ZrO2, compared with Mineral Trioxide Aggregate (MTA). According to ISO 10993-12:2012 (E) recommendations, 0.2 g of each cement were applied in transwell inserts and placed in 24-well culture plates containing 1 mL of culture medium (DMEM). After 24 h of incubation, the extracts (DMEM containing components released from the cements) were applied to immortalized odontoblast-like MDPC-23 cells. Cell viability (MTT test), alkaline phosphatase activity (ALP), total protein production and cell morphology (Scanning Electron Microscopy - SEM) were evaluated. The volume of 50 µL of extract was used to determine the chemical elements released by the cements using Energy Dispersive Spectroscopy (EDS). The following groups were established (n=6): NC - negative control (without treatment); EB - EndoBinder without radiopacifier; EBBO - EndoBinder+Bi2O3; EBZnO - EndoBinder+ZnO; EBZrO - EndoBinder+ZrO2 and WMTA - White MTA. Data were subjected to statistical analysis (Kruskal-Wallis test, level of significance=5%). Cells exposed to the different versions of EndoBinder presented small reduction in viability, total protein production and ALP activity, with values similar to the NC and WMTA groups (p>0.05). Different elements (C, O, Na, Al, P, Si, Cl, Bi, K) released by the cements were detected in the extracts. However, the cells had no significant changes in their morphology. EndoBinder and MTA did not affect negatively the metabolism of the odontoblastic-like cells, showing it to be cytocompatible, irrespective of the used radiopacifier.
本研究旨在评估含不同射线阻射剂（Bi2O3、ZnO或ZrO2）的铝酸钙水泥（EndoBinder）与矿物三氧化物凝聚体（MTA）相比的细胞毒性。根据ISO 10993 - 12:2012（E）的建议，将0.2 g每种水泥应用于Transwell小室插入物中，并置于含有1 mL培养基（DMEM）的24孔培养板中。孵育24小时后，将提取物（含有从水泥中释放成分的DMEM）应用于永生化的成牙本质细胞样MDPC - 23细胞。评估细胞活力（MTT试验）、碱性磷酸酶活性（ALP）、总蛋白产量和细胞形态（扫描电子显微镜 - SEM）。使用50 μL提取物的体积，通过能量色散光谱（EDS）测定水泥释放的化学元素。设立以下组（n = 6）：NC - 阴性对照（未处理）；EB - 不含射线阻射剂的EndoBinder；EBBO - EndoBinder + Bi2O3；EBZnO - EndoBinder + ZnO；EBZrO - EndoBinder + ZrO2和WMTA - 白色MTA。数据进行统计分析（Kruskal - Wallis检验，显著性水平 = 5%）。暴露于不同版本EndoBinder的细胞在活力、总蛋白产量和ALP活性方面呈现小幅下降，其值与NC组和WMTA组相似（p>0.05）。在提取物中检测到水泥释放的不同元素（C、O、Na、Al、P、Si、Cl、Bi、K）。然而，细胞形态没有显著变化。EndoBinder和MTA对成牙本质细胞样细胞的代谢没有负面影响，表明无论使用何种射线阻射剂，它们都具有细胞相容性。
