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人子宫颈癌组织和非癌组织中用于RT-qPCR数据标准化的最佳内参基因的鉴定

Identification of Optimal Reference Genes for Normalization of RT-qPCR Data in Cancerous and Non-Cancerous Tissues of Human Uterine Cervix.

作者信息

Tan Shing Cheng, Ismail Mohd Pazudin, Duski Daniel Roza, Othman Nor Hayati, Bhavaraju Venkata Murali Krishna, Ankathil Ravindran

机构信息

a Human Genome Centre, School of Medical Sciences, Health Campus , Universiti Sains Malaysia , Kelantan , Malaysia.

b Department of Obstetrics and Gynecology , School of Medical Sciences, Health Campus, Universiti Sains Malaysia , Kelantan , Malaysia.

出版信息

Cancer Invest. 2017 Mar 16;35(3):163-173. doi: 10.1080/07357907.2017.1278767. Epub 2017 Feb 17.

DOI:10.1080/07357907.2017.1278767
PMID:28301252
Abstract

This study aimed to identify the most stably expressed reference genes from a panel of 32 candidate genes for normalization of reverse transcription-quantitative real-time polymerase chain reaction data in cancerous and non-cancerous tissues of human uterine cervix. Overall, PUM1, YWHAZ, and RPLP0 were identified as the most stably expressed genes in paired cancerous and non-cancerous tissues. The results were further stratified by the state of malignancy of the tissues, histopathological type of the cancer, and the human papillomavirus-type.

摘要

本研究旨在从32个候选基因中鉴定出最稳定表达的内参基因，用于标准化人子宫颈癌组织和癌旁组织中逆转录-定量实时聚合酶链反应数据。总体而言，PUM1、YWHAZ和RPLP0被确定为配对癌组织和癌旁组织中最稳定表达的基因。结果进一步按组织的恶性程度、癌症的组织病理学类型和人乳头瘤病毒类型进行分层。

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