Lin G Y, Du X L, Shan J J, Zhang Y N, Zhang Y Q, Wang Q H
Blood Transfusion Department, People's Liberation Army 474th Hospital, Urumqi, Xinjiang Uyghur Autonomous Region, China
Blood Transfusion Department, People's Liberation Army 474th Hospital, Urumqi, Xinjiang Uyghur Autonomous Region, China.
Genet Mol Res. 2017 Mar 15;16(1):gmr-16-01-gmr.16019176. doi: 10.4238/gmr16019176.
Human blood groups are a significant resource for patients, leading to a fierce international competition in the screening of rare blood groups. Some rare blood group screening programs have been implemented in western countries and Japan, but not particularly in China. Recently, the genetic background of ABO and Rh blood groups for different ethnic groups or regions in China has been focused on increasingly. However, rare blood groups such as MN, Duffy, Kidd, MNS, and Diego are largely unexplored. No systematic reports exist concerning the polymorphisms and allele frequencies of rare blood groups in China's ethnic minorities such as Uygur and Kazak populations of Xinjiang, unlike those on the Han population. Therefore, this study aimed to investigate the allele frequencies of rare blood groups, namely, MNS, Duffy, Kell, Dombrock, Diego, Kidd, Scianna, Colton, and Lutheran in the Uygur population of Xinjiang Single specific primer-polymerase chain reaction was performed for genotyping and statistical analysis of 9 rare blood groups in 158 Uygur individuals. Allele frequencies were compared with distribution among other ethnic groups. Observed and expected values of genotype frequencies were compared using the chi-square test. Genotype frequencies obeyed the Hardy-Weinberg equilibrium (P > 0.5) and allele frequencies were stable. Of all subjects detected, 4 cases carried the rare phenotype Ss of MNS blood group (frequency of 0.0253), and 1 case carried the phenotype Jk (frequency of 0.0063). Frequencies of the four groups, MNS, Duffy, Dombrock, and Diego, in the Uygur population differed from those in other ethnic groups. Gene distribution of the Kell, Kidd, and Colton was similar to that in Tibetan and Han populations, though there were some discrepancies. Gene distribution of Scianna and Lutheran groups showed monomorphism similar to that in Tibetan and Han populations. These findings could contribute to the investigation of the origin, evolution, and hematology of Uygur population of Xinjiang and assist in screening of rare blood groups in ethnic minorities, meeting of clinical blood supply demands, and building of the national rare blood group library.
人类血型对患者来说是一项重要资源，这导致了在稀有血型筛查方面的激烈国际竞争。西方国家和日本已经实施了一些稀有血型筛查项目，但在中国并非如此。最近，中国不同民族或地区的ABO和Rh血型的遗传背景越来越受到关注。然而，MN、达菲、基德、MNS和迭戈等稀有血型在很大程度上尚未得到充分研究。与汉族不同，关于中国少数民族如新疆维吾尔族和哈萨克族稀有血型的多态性和等位基因频率，目前尚无系统报道。因此，本研究旨在调查新疆维吾尔族人群中MNS、达菲、凯尔、多姆布罗克、迭戈、基德、斯恰纳、科尔顿和路德等稀有血型的等位基因频率。对158名维吾尔族个体的9种稀有血型进行单特异性引物聚合酶链反应基因分型和统计分析。将等位基因频率与其他民族的分布情况进行比较。使用卡方检验比较基因型频率观测值和预期值。基因型频率符合哈迪-温伯格平衡（P>0.5），等位基因频率稳定。在所有检测对象中，有4例携带MNS血型的稀有表型Ss（频率为0.0253），1例携带Jk表型（频率为0.0063）。维吾尔族人群中MNS、达菲、多姆布罗克和迭戈这四组血型的频率与其他民族不同。凯尔、基德和科尔顿血型基因分布与藏族和汉族人群相似，但也存在一些差异。斯恰纳和路德血型组的基因分布呈现出与藏族和汉族人群相似相同型。这些研究结果有助于对新疆维吾尔族人群的起源、进化和血液学进行研究，并有助于少数民族稀有血型的筛查、满足临床血液供应需求以及建立国家稀有血型库。
