[ω-3多不饱和脂肪酸对脓毒症大鼠淋巴细胞凋亡率的影响]
[Effects of ω-3 polyunsaturated fatty acids on lymphocyte apoptosis rate in rats with sepsis].
作者信息
Miao Hong-Jun, Wang Dan, Ge Xu-Hua, Li Xiao-Nan
机构信息
Emergency Department, Children's Hospital of Nanjing Medical University, Nanjing 210008, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2017 Mar;19(3):355-360. doi: 10.7499/j.issn.1008-8830.2017.03.021.
OBJECTIVE
To investigate the effects of ω-3 polyunsaturated fatty acids (ω-3 PUFAs) on the apoptosis of thymic and splenic lymphocytes in rats with sepsis.
METHODS
A total of 80 female Sprague-Dawley rats aged 7-8 weeks were randomly divided into model group, conventional lipid emulsion group (0.1 g/kg daily), low-dose ω-3 PUFAs group (0.1 g/kg daily), middle-dose ω-3 PUFAs group (0.2 g/kg daily), and high-dose ω-3 PUFAs group (0.3 g/kg daily). Cecal ligation and puncture were used to establish a rat model of sepsis. The treatment groups were then given tail vein injection of lipid emulsion or glucose diluents of ω-3 PUFAs at different doses, and the model group was given glucose injection via the tail vein at the same dose. According to the time of sacrifice, each group was further divided into 24-hour and 72-hour subgroups, with 8 rats in each subgroup. Hematoxylin and eosin staining was used to observe the pathological changes in the thymus and spleen. TUNEL was used to measure the apoptosis rates of thymic and splenic lymphocytes.
RESULTS
In the three ω-3 PUFAs groups, the rats had a complete thymic lobular structure and clear structures of the cortex and medulla. In the model and the conventional lipid emulsion groups, the boundaries of the cortex and medulla were unclear and the number of lymphocytes was significantly reduced. In the ω-3 PUFAs groups, the structure of the red and white pulp of the spleen was maintained with the presence of splenic follicles, while in the model and the conventional lipid emulsion groups, the structure of the red and white pulp of the spleen was disordered and splenic follicles were significantly reduced or disappeared. Compared with the model and the conventional lipid emulsion groups, the ω-3 PUFAs groups showed significant reductions in the apoptosis rates of thymic and splenic lymphocytes at 24 and 72 hours (P<0.01). Compared with the low-dose ω-3 PUFAs group, the high-dose ω-3 PUFAs group had significantly reduced apoptosis rates of splenic lymphocytes at 24 and 72 hours (P<0.05).
CONCLUSIONS
ω-3 PUFAs can reduce the apoptosis of thymic and splenic lymphocytes in rats with sepsis in a dose-dependent manner.
目的
探讨ω-3多不饱和脂肪酸（ω-3 PUFAs）对脓毒症大鼠胸腺和脾脏淋巴细胞凋亡的影响。
方法
选取80只7-8周龄雌性Sprague-Dawley大鼠，随机分为模型组、常规脂质乳剂组（每日0.1 g/kg）、低剂量ω-3 PUFAs组（每日0.1 g/kg）、中剂量ω-3 PUFAs组（每日0.2 g/kg）和高剂量ω-3 PUFAs组（每日0.3 g/kg）。采用盲肠结扎穿孔法建立大鼠脓毒症模型。然后对各治疗组经尾静脉注射不同剂量的脂质乳剂或ω-3 PUFAs葡萄糖稀释液，模型组经尾静脉注射相同剂量的葡萄糖注射液。根据处死时间，每组再分为24小时和72小时亚组，每个亚组8只大鼠。采用苏木精-伊红染色观察胸腺和脾脏的病理变化。采用TUNEL法检测胸腺和脾脏淋巴细胞的凋亡率。
结果
在三个ω-3 PUFAs组中，大鼠胸腺小叶结构完整，皮质和髓质结构清晰。在模型组和常规脂质乳剂组中，皮质和髓质边界不清，淋巴细胞数量明显减少。在ω-3 PUFAs组中，脾脏红髓和白髓结构保持，有脾小结存在，而在模型组和常规脂质乳剂组中，脾脏红髓和白髓结构紊乱，脾小结明显减少或消失。与模型组和常规脂质乳剂组相比，ω-3 PUFAs组在24小时和72小时时胸腺和脾脏淋巴细胞凋亡率显著降低（P<0.01）。与低剂量ω-3 PUFAs组相比，高剂量ω-3 PUFAs组在24小时和72小时时脾脏淋巴细胞凋亡率显著降低（P<0.05）。
结论
ω-3 PUFAs可剂量依赖性降低脓毒症大鼠胸腺和脾脏淋巴细胞的凋亡。
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