静脉注射ω-3和ω-6脂肪乳剂对接受全胃肠外营养的烧伤大鼠细胞因子产生及迟发型超敏反应的影响。

Effects of intravenous omega-3 and omega-6 fat emulsion on cytokine production and delayed type hypersensitivity in burned rats receiving total parenteral nutrition.

作者信息

Hayashi N, Tashiro T, Yamamori H, Takagi K, Morishima Y, Otsubo Y, Sugiura T, Furukawa K, Nitta H, Nakajima N, Suzuki N, Ito I

机构信息

First Department of Surgery, Chiba University, School of Medicine, Japan.

出版信息

JPEN J Parenter Enteral Nutr. 1998 Nov-Dec;22(6):363-7. doi: 10.1177/0148607198022006363.

DOI:10.1177/0148607198022006363

PMID:9829609

Abstract

BACKGROUND

The effects of fat emulsions containing omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) on fatty acid profile, cytokine production, and delayed type hypersensitivity (DTH) in burned rats receiving total parenteral nutrition (TPN) were investigated.

METHODS

A fat emulsion containing only eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) was prepared (omega-3 fat emulsion). Sprague-Dawley rats were fed fat-free chow for 2 weeks and were divided into three groups according to the contents of TPN. Groups C (n = 11) and C' (n = 14) received fat-free TPN. Group omega6 received safflower oil emulsion containing linoleic acid (omega-6 PUFA) (n = 11). Group omega3 (n = 11) received safflower oil emulsion (19% of total caloric intake) and fat emulsion containing only EPA and DHA (1% of total calories). On day 5, all rats except for those in group C' were subjected to a 20% full-thickness burn. Group C' did not receive burns. After 48 hours, the rats were killed.

RESULTS

The interleukin (IL)-8 concentration was significantly 45% lower in group omega3 than in group C (p < .05). The IL-10 concentration was significantly 15% lower in group omega3 than in group omega6 (p < .05). The IL-6 concentration was increased in group omega6 but not in group omega3 when compared with group C. The IL-6 and IL-8 were not detected in group C'. Prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) concentrations were increased by burn injury, but there were no significant differences among the burned groups. Cell-mediated immunity was thus significantly decreased in burned groups (groups C, omega6, and omega3; p < .01). However, the decrease of DTH was smaller in group omega3 and significantly greater when compared with groups C and omega6 (p < .05).

CONCLUSIONS

omega-6 PUFAs increased serum inflammatory cytokine levels in a stressed state. omega-3 fat emulsion reduced IL-8 and IL-10 levels and prevented immunosuppression in burned rats that were receiving TPN.

摘要

背景

研究了含ω-3和ω-6多不饱和脂肪酸（PUFAs）的脂肪乳剂对接受全胃肠外营养（TPN）的烧伤大鼠脂肪酸谱、细胞因子产生及迟发型超敏反应（DTH）的影响。

方法

制备仅含二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的脂肪乳剂（ω-3脂肪乳剂）。将Sprague-Dawley大鼠喂饲无脂饲料2周，并根据TPN的成分分为三组。C组（n = 11）和C'组（n = 14）接受无脂TPN。ω6组接受含亚油酸（ω-6 PUFA）的红花油乳剂（n = 11）。ω3组（n = 11）接受红花油乳剂（占总热量摄入的19%）和仅含EPA和DHA的脂肪乳剂（占总热量的1%）。在第5天，除C'组外的所有大鼠均接受20%的全层烧伤。C'组未接受烧伤。48小时后，处死大鼠。

结果

ω3组白细胞介素（IL）-8浓度比C组显著低45%（p < 0.05）。ω3组IL-10浓度比ω6组显著低15%（p < 0.05）。与C组相比，ω6组IL-6浓度升高，而ω3组未升高。C'组未检测到IL-6和IL-8。烧伤损伤使前列腺素E2（PGE2）和血栓素B2（TXB2）浓度升高，但烧伤组之间无显著差异。因此，烧伤组（C组、ω6组和ω3组）的细胞介导免疫显著降低（p < 0.01）。然而，ω3组DTH的降低较小，与C组和ω6组相比显著更大（p < 0.05）。