Hayashi N, Tashiro T, Yamamori H, Takagi K, Morishima Y, Otsubo Y, Sugiura T, Furukawa K, Nitta H, Nakajima N, Suzuki N, Ito I
First Department of Surgery, Chiba University, School of Medicine, Japan.
JPEN J Parenter Enteral Nutr. 1998 Nov-Dec;22(6):363-7. doi: 10.1177/0148607198022006363.
The effects of fat emulsions containing omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) on fatty acid profile, cytokine production, and delayed type hypersensitivity (DTH) in burned rats receiving total parenteral nutrition (TPN) were investigated.
A fat emulsion containing only eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) was prepared (omega-3 fat emulsion). Sprague-Dawley rats were fed fat-free chow for 2 weeks and were divided into three groups according to the contents of TPN. Groups C (n = 11) and C' (n = 14) received fat-free TPN. Group omega6 received safflower oil emulsion containing linoleic acid (omega-6 PUFA) (n = 11). Group omega3 (n = 11) received safflower oil emulsion (19% of total caloric intake) and fat emulsion containing only EPA and DHA (1% of total calories). On day 5, all rats except for those in group C' were subjected to a 20% full-thickness burn. Group C' did not receive burns. After 48 hours, the rats were killed.
The interleukin (IL)-8 concentration was significantly 45% lower in group omega3 than in group C (p < .05). The IL-10 concentration was significantly 15% lower in group omega3 than in group omega6 (p < .05). The IL-6 concentration was increased in group omega6 but not in group omega3 when compared with group C. The IL-6 and IL-8 were not detected in group C'. Prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) concentrations were increased by burn injury, but there were no significant differences among the burned groups. Cell-mediated immunity was thus significantly decreased in burned groups (groups C, omega6, and omega3; p < .01). However, the decrease of DTH was smaller in group omega3 and significantly greater when compared with groups C and omega6 (p < .05).
omega-6 PUFAs increased serum inflammatory cytokine levels in a stressed state. omega-3 fat emulsion reduced IL-8 and IL-10 levels and prevented immunosuppression in burned rats that were receiving TPN.
研究了含ω-3和ω-6多不饱和脂肪酸（PUFAs）的脂肪乳剂对接受全胃肠外营养（TPN）的烧伤大鼠脂肪酸谱、细胞因子产生及迟发型超敏反应（DTH）的影响。
制备仅含二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的脂肪乳剂（ω-3脂肪乳剂）。将Sprague-Dawley大鼠喂饲无脂饲料2周，并根据TPN的成分分为三组。C组（n = 11）和C'组（n = 14）接受无脂TPN。ω6组接受含亚油酸（ω-6 PUFA）的红花油乳剂（n = 11）。ω3组（n = 11）接受红花油乳剂（占总热量摄入的19%）和仅含EPA和DHA的脂肪乳剂（占总热量的1%）。在第5天，除C'组外的所有大鼠均接受20%的全层烧伤。C'组未接受烧伤。48小时后，处死大鼠。
ω3组白细胞介素（IL）-8浓度比C组显著低45%（p < 0.05）。ω3组IL-10浓度比ω6组显著低15%（p < 0.05）。与C组相比，ω6组IL-6浓度升高，而ω3组未升高。C'组未检测到IL-6和IL-8。烧伤损伤使前列腺素E2（PGE2）和血栓素B2（TXB2）浓度升高，但烧伤组之间无显著差异。因此，烧伤组（C组、ω6组和ω3组）的细胞介导免疫显著降低（p < 0.01）。然而，ω3组DTH的降低较小，与C组和ω6组相比显著更大（p < 0.05）。
ω-6 PUFAs在应激状态下会升高血清炎症细胞因子水平。ω-3脂肪乳剂可降低IL-8和IL-10水平，并预防接受TPN的烧伤大鼠的免疫抑制。
