Inoue T, Suzuki S
National Research Institute of Police Science, Tokyo, Japan.
J Chromatogr. 1987 Nov 27;422:197-204. doi: 10.1016/0378-4347(87)80452-8.
A high-performance liquid chromatographic method for the determination of triazolam and its main metabolites (1-hydroxymethyltriazolam and 4-hydroxytriazolam) in human urine has been developed. After hydrolysis with beta-glucuronidase, the unchanged drug and its metabolites were extracted with a Sep-Pak C18 cartridge and further purified by a Sep-Pak silica cartridge. The extract was chromatographed on a reversed-phase column using a mobile phase consisting of methanol-10 mM phosphate buffer, pH 8 (65:35) and UV detection at 220 nm. The overall recoveries of triazolam, 1-hydroxymethyltriazolam and 4-hydroxytriazolam were ca. 88, 75 and 75%, respectively, and the detection limits of these compounds were 5 ng/ml, using a 10-ml specimen.
已开发出一种高效液相色谱法，用于测定人尿中三唑仑及其主要代谢物（1-羟甲基三唑仑和4-羟基三唑仑）。用β-葡萄糖醛酸酶水解后，用Sep-Pak C18柱提取未变化的药物及其代谢物，并用Sep-Pak硅胶柱进一步纯化。提取物在反相柱上进行色谱分析，流动相为甲醇-10 mM磷酸盐缓冲液，pH 8（65:35），在220 nm处进行紫外检测。使用10 ml样本时，三唑仑、1-羟甲基三唑仑和4-羟基三唑仑的总回收率分别约为88%、75%和75%，这些化合物的检测限为5 ng/ml。
