光遗传学与系统神经科学中互补方法的整合。
Integration of optogenetics with complementary methodologies in systems neuroscience.
作者信息
Kim Christina K, Adhikari Avishek, Deisseroth Karl
机构信息
Neurosciences Program, Stanford University, 318 Campus Drive, Stanford, California 94305, USA.
Department of Bioengineering, Stanford University, 443 Via Ortega, Stanford, California 94305, USA.
出版信息
Nat Rev Neurosci. 2017 Mar 17;18(4):222-235. doi: 10.1038/nrn.2017.15.
Modern optogenetics can be tuned to evoke activity that corresponds to naturally occurring local or global activity in timing, magnitude or individual-cell patterning. This outcome has been facilitated not only by the development of core features of optogenetics over the past 10 years (microbial-opsin variants, opsin-targeting strategies and light-targeting devices) but also by the recent integration of optogenetics with complementary technologies, spanning electrophysiology, activity imaging and anatomical methods for structural and molecular analysis. This integrated approach now supports optogenetic identification of the native, necessary and sufficient causal underpinnings of physiology and behaviour on acute or chronic timescales and across cellular, circuit-level or brain-wide spatial scales.
现代光遗传学可以进行调整，以激发在时间、幅度或单细胞模式上与自然发生的局部或全局活动相对应的活动。这一成果不仅得益于过去10年光遗传学核心特征的发展（微生物视蛋白变体、视蛋白靶向策略和光靶向装置），还得益于最近光遗传学与互补技术的整合，这些技术涵盖了电生理学、活动成像以及用于结构和分子分析的解剖学方法。这种综合方法现在支持在急性或慢性时间尺度上以及跨细胞、回路水平或全脑空间尺度，对生理和行为的天然、必要和充分因果基础进行光遗传学鉴定。
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