Weirich G, Karlson P
Physiologisch-Chemisches Institut der Philipps-Universität, Marburg a. d. Lahn, Germany.
Wilhelm Roux Arch Entwickl Mech Org. 1969 Jun;164(2):170-181. doi: 10.1007/BF00581997.
The distribution of tritium-labelled ecdysone in salivary glands ofChironomus tentans and different tissues ofRhynchosciara spec, has been studied, following in vivo incubations and either of two modified ethanol-acetic acid fixation techniques. The results of autoradiographic analysis show maximum label over the cytoplasm or evenly labelled cytoplasm and nuclei, within a wide range of incubation times. Indications of distinct localization at chromosomal loci are not conclusive. There is a remarkably high amount of radioactivity in the lumen of the anterior intestine as soon as six minutes after hormone injection. The limitations of histoautoradiographic studies in attempts to localize the cellular receptor of ecdysone are discussed.
在体内孵育后，采用两种改良的乙醇 - 乙酸固定技术之一，研究了氚标记蜕皮激素在摇蚊（Chironomus tentans）唾液腺和大头金蝇（Rhynchosciara spec）不同组织中的分布。放射自显影分析结果表明，在广泛的孵育时间范围内，细胞质上有最大标记或细胞质和细胞核均匀标记。关于在染色体位点的明显定位迹象尚无定论。激素注射后仅6分钟，前肠腔内就有非常高的放射性。讨论了组织放射自显影研究在定位蜕皮激素细胞受体方面的局限性。
