[Influence of inhibitors of RNA and protein synthesis and inductors upon cell affinity of amphibian tissues].

Isolated Amphibian gastrula ectoderm, pretreated in different ways, was combined with endoderm. 1. Untreated ectoderm separates from endoderm after several days. 2. Ectoderm, treated with the vegetalizing inducing factor, is surrounded by endoderm, which spreads over the treated ectoderm as an uni- or multicellular layer. 3. Treatment of ectoderm with actidion (2 Μg/ml for 5 h or 1 Μg/ml for 20 h), an inhibitor of protein synthesis, prior to induction prevents the spreading of endoderm over ectoderm. 4. Treatment of ectoderm with AcD, an inhibitor of RNA synthesis, prior to induction does not prevent the spreading of endoderm over ectoderm. 5. Treatment of ectoderm with AcD without inductor changes the affinity of ectoderm to endoderm. Ectoderm, treated with 1 Μg/ml AcD, spreads over the endoderm as a thin layer. After treatment with 2 Μg/ml AcD, in some cases endoderm spreads over the ectoderm.