Grunz Horst, Tiedemann Hildegard
Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Berlin, Germany.
Wilehm Roux Arch Dev Biol. 1977 Sep;181(3):261-265. doi: 10.1007/BF00848425.
Isolated amphibian (Triturus alpestris) gastrula ectoderm was treated with cyclic nucleotides for 24 h and cultured up to 12 days. Explants treated with$cyclic N-Monobutyryl-adenosine-3'∶5'-monophosphate, cyclic Dibutyryladenosine-3'∶5'-monophosphate and cyclic Dibutyrylguanosine-3'∶5'-monophosphate in a concentration of 10 and 10 M did not differentiate into mesoderm- or endoderm-derived tissues. The number of explants with small neural and neuroid structures did not exceed the percentage found in the control series. Inductions could also not be obtained when ectoderm was dissociated prior to the treatment with cyclic nucleotides, or when theophylline (which inhibits phosphodiesterase) was added to the culture medium. The results are discussed with regard to the possible mode of action of the vegetalizing factor.
将分离出的蝾螈原肠胚外胚层用环核苷酸处理24小时，并培养12天。用浓度为10⁻⁴和10⁻⁵M的环单丁酰腺苷-3':5'-单磷酸、环二丁酰腺苷-3':5'-单磷酸和环二丁酰鸟苷-3':5'-单磷酸处理的外植体未分化为中胚层或内胚层来源的组织。具有小神经和神经样结构的外植体数量不超过对照系列中的百分比。当外胚层在用环核苷酸处理之前解离，或者当将茶碱（抑制磷酸二酯酶）添加到培养基中时，也无法获得诱导。针对植物化因子可能的作用方式对结果进行了讨论。
