Allen R D, Cohen E A, Vonder Haar R A, Adams C A, Ma D P, Nessler C L, Thomas T L
Biology Department, Texas A & M University, College Station 77843.
Mol Gen Genet. 1987 Dec;210(2):211-8. doi: 10.1007/BF00325686.
The complete sequence of a sunflower (Helianthus annuus) gene, HaG5, encoding a 2 S albumin storage protein was determined. The predicted unprocessed precursor has 295 amino acids, is rich in glutamine residues (24%) and contains a hydrophobic amino-terminus that is similar to the consensus signal peptide. Amino acid sequencing of the mature protein revealed extensive post-translational processing. Nuclease protection and primer extension analysis indicated a major transcriptional start 30 nucleotides 5' of the predicted ATG start codon. Additional sequence data, determined from a nearly full length cDNA recombinant, indicate that HaG5 is a member of a small gene family comprised of at least two divergent genes. Comparison of the predicted HaG5 gene product with sequences of other known plant proteins revealed distant but significant homology with the napins of Brassica and other heterogeneous seed proteins in the albumin superfamily.
测定了向日葵（Helianthus annuus）中一个编码2S白蛋白储存蛋白的基因HaG5的完整序列。预测的未加工前体有295个氨基酸，富含谷氨酰胺残基（24%），并含有一个与共有信号肽相似的疏水氨基末端。成熟蛋白的氨基酸测序显示有广泛的翻译后加工。核酸酶保护和引物延伸分析表明，在预测的ATG起始密码子5'端30个核苷酸处有一个主要转录起始位点。从一个几乎全长的cDNA重组体确定的其他序列数据表明，HaG5是一个由至少两个不同基因组成的小基因家族的成员。将预测的HaG5基因产物与其他已知植物蛋白的序列进行比较，发现与芸苔属的napin蛋白及白蛋白超家族中的其他异种种子蛋白有远缘但显著的同源性。
