Cho P, Davenport R, Whitt G S, Booth G M, Larsen J R
Department of Zoology, University of Illinois, USA.
Department of Entomology, University of Illinois, 61801, Urbana, Illinois.
Wilhelm Roux Arch Entwickl Mech Org. 1972 Sep;170(3):209-220. doi: 10.1007/BF01380677.
The synthesis of esterases was analysed in the ovariole of the milkweed bug using electrophoretic and histochemical methods. We found a total of 12 esterases from whole ovaries. Using the inhibitors p-hydroxymercuribenzoate, diisopropyl phosphorofluoridate and eserine, 3 of the bands were classed as acetylesterases, 7 carboxylesterases, and 2 arylesterases. No change in esterase patterns occurred during oogenesis.The esterase activity in the follicular epithelium was primarily arylesterase and carboxylesterase. A carboxylesterase was also identified from the central region of the follicle cell. The yolk cells contained mainly carboxylesterase. There was an apparent activation of enzyme activity following pre-treatment with eserine.
采用电泳和组织化学方法分析了乳草蝽卵巢管中酯酶的合成情况。我们从整个卵巢中总共发现了12种酯酶。使用对羟基汞苯甲酸酯、二异丙基氟磷酸酯和毒扁豆碱等抑制剂，其中3条带被归类为乙酰酯酶，7条为羧酸酯酶，2条为芳基酯酶。在卵子发生过程中酯酶模式没有变化。卵泡上皮中的酯酶活性主要是芳基酯酶和羧酸酯酶。在卵泡细胞的中央区域也鉴定出一种羧酸酯酶。卵黄细胞主要含有羧酸酯酶。用毒扁豆碱预处理后，酶活性有明显激活。
