Brugal G
Laboratoire de Zoologie, Institut de Recherches Biologiques, Université Scientifique et Médicale de Grenoble, France.
Wilhelm Roux Arch Entwickl Mech Org. 1971 Sep;168(3):205-225. doi: 10.1007/BF00634064.
We observed in Pleurodeles embryos, stage 34, that the duration of the cell cycle and its phases was approximately the same for every tissue but was easily modified by varying the temperature. The generation time and the duration of S phase in embryos submitted to a 12° C temperature instead of 26° C are tripled or quadrupled. A temperature rise produced a proportionale shortening inG andM phases and a lengthening inG phase. ThisG phase is not detectable at 12° C but represent a 1/4 of the total generation time at 26° C. The more differentiated the cells are, the longer is theG time. The cell population studied during these experiments are growing exponentially. Growth fraction, which represents the exponential growth basis, is temperature independent but has a tissue specificity. This growth fraction is smaller the more the tissue is differentiated. However, the relative rate of cell division, inversely proportional to the generation time, is temperature dependent and appears to control the embryo's relative rate of growth under different temperatures.
我们在有尾两栖类胚胎的34期观察到，每个组织的细胞周期及其各阶段的持续时间大致相同，但很容易因温度变化而改变。与处于26℃相比，处于12℃的胚胎的世代时间和S期持续时间增加了两倍或三倍。温度升高会使G期和M期成比例缩短，G期延长。在12℃时无法检测到该G期，但在26℃时它占总世代时间的1/4。细胞分化程度越高，G期时间越长。在这些实验中研究的细胞群体呈指数增长。代表指数增长基础的生长分数与温度无关，但具有组织特异性。组织分化程度越高，该生长分数越小。然而，与世代时间成反比的细胞分裂相对速率与温度有关，并且似乎控制着胚胎在不同温度下的相对生长速率。
