RAO P N, ENGELBERG J
Science. 1965 May 21;148(3673):1092-4. doi: 10.1126/science.148.3673.1092.
After a shift in temperature, the population kinetics of HeLa cells does not immediately reach a steady state characteristic of the new temperature. During the transition period the duration of each period of the life cycle (period before DNA synthesis, of DNA synthesis, after DNA synthesis, and the period of mitosis) increases toward its steady-state value. Of the four periods, the period before DNA synthesis reaches its steady-state value fastest. Exponential growth can be maintained only from 33 degrees C through 40 degrees C. The period of mitosis is the most temperature-sensitive period of the life cycle of the cell. At subnormal temperatures (26 degrees C through 31 degrees C) there is an accumulation of cells in mitosis, and mitotic indices as high as 0.44 can be obtained. The duration of mitosis is a function both of the temperature and of the time which the cell has spent at this temperature.
温度变化后，HeLa细胞的群体动力学不会立即达到新温度所特有的稳态。在过渡期，生命周期各阶段（DNA合成前期、DNA合成期、DNA合成后期和有丝分裂期）的持续时间朝着其稳态值增加。在这四个阶段中，DNA合成前期最快达到其稳态值。指数生长仅在33摄氏度至40摄氏度之间可以维持。有丝分裂期是细胞生命周期中对温度最敏感的时期。在亚正常温度（26摄氏度至31摄氏度）下，有丝分裂期会有细胞积累，并且可以获得高达０.44的有丝分裂指数。有丝分裂的持续时间既是温度的函数，也是细胞在该温度下所经历时间的函数。
