Poodry Clifton A, Schneiderman Howard A
Developmental Biology Laboratory and Center for Pathobiology, University of California Irvine, 92664, Irvine, California.
Dept. of Zoology, University of British Columbia, Vancouver 8 British Columbia, Canada.
Wilhelm Roux Arch Entwickl Mech Org. 1971 Mar;168(1):1-9. doi: 10.1007/BF00582000.
Leg and wing imaginal discs of mature larvae ofDrosophila melanogaster when treated with 0.1% trypsin for 5-10 min underwent a change in shape that closely resembled normal pupal morphogenesis. Simultaneously, the cells of the disc epithelium changed in shape from tall columnar to cuboidal. Colcemid eliminated microtubules but was without effect on the shape of the imaginal discs or their cells. Tryptic digestion reduced non-junctional intercellular adhesivity but septate desmosomes and gap junctions remained intact.It is proposed that the structure of imaginal discs permits the packaging of the anlagen of the adult integument so that they can change shape and replace the larval structures in a brief period. Apparently most of the definitive form of the pupal leg is built into the disc and becomes visible within a few minutes as intercellular adhesivity is changed.
用0.1%胰蛋白酶处理5 - 10分钟后，黑腹果蝇成熟幼虫的腿和翅成虫盘发生了形状变化，这与正常蛹的形态发生非常相似。同时，盘上皮细胞的形状从高柱状变为立方状。秋水仙酰胺消除了微管，但对成虫盘及其细胞的形状没有影响。胰蛋白酶消化降低了非连接性细胞间粘附性，但分隔连接和间隙连接保持完整。有人提出，成虫盘的结构允许成虫体表原基进行包装，以便它们能够在短时间内改变形状并取代幼虫结构。显然，蛹腿的大部分最终形态在盘中就已形成，并在细胞间粘附性改变后的几分钟内显现出来。
